rsmH

rsmH
168

SAM-dependent 16S rRNA m4C1402 methyltransferase

Locus
BSU_15140
Molecular weight
35.15 kDa
Isoelectric point
6.03
Protein length
311 aaSequenceBlast
Gene length
936 bpSequenceBlast
Function
fine-tuning of the ribosomal decoding center
Product
Ribosomal RNA small subunit methyltransferase H
Essential
no
E.C.
2.1.1.199
Synonyms
mraW, yllC, ylxA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0275 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,580,622  1,581,557
The protein
Catalyzed reaction/ biological activity
cytidine1402 in 16S rRNA + S-adenosyl-L-methionine --> H+ + N4-methylcytidine1402 in 16S rRNA + S-adenosyl-L-homocysteine (according to UniProt)
Protein family
Methyltransferase superfamily (according to UniProt)
Structure
3TKA (PDB) (from Escherichia coli, 46% identity) PubMed
1N2X (PDB) (from Thermotoga maritima, 41% identity, 70% similarity) PubMed
Q07876 (AlphaFold)
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
mraZ-rsmH-ftsL-pbpB
Description
PubMed
Regulation
constitutively expressed PubMed
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mraZ→pbpB

2025-04-09 04:33:25

Jstuelk

137

d5321fe6b1e9387ca670f35171261c39867c1f3d

9D42077BFFD1B3A740698D26FB54D7B31E3FD0C7

Additional information
the mRNA is cleaved by RNase III PubMed
Biological materials
Mutant
MGNA-B130 (ylxA::erm), available at the NBRP B. subtilis, Japan
1A818 ( mraW::erm), PubMed, available at BGSC
BKE15140 (rsmH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTGTGTTGGTCCCACC,  downstream forward: _UP4_TAACGAAACGCGAACGCAAA
BKK15140 (rsmH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTGTGTTGGTCCCACC,  downstream forward: _UP4_TAACGAAACGCGAACGCAAA
References
Reviews
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Original Publications
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1F894D6895CE13AA006CFE13A702E46C568F6E79

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Time of last update: 2025-04-11 16:01:49

Author of last update: Jstuelk