ThiV

• Description: thiamine ABC transporter (membrane protein)
  
  

Gene name	thiV
Synonyms	ykoE
Essential	no
Product	thiamine ABC transporter (membrane protein)
Function	thiamine uptake
Gene expression levels in SubtiExpress: thiV
Interactions involving this protein in SubtInteract: ThiV
Metabolic function and regulation of this protein in SubtiPathways: thiV
MW, pI	21 kDa, 9.89
Gene length, protein length	597 bp, 199 aa
Immediate neighbours	thiW, thiU
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, biosynthesis of cofactors, membrane proteins

This gene is a member of the following regulons

Thi-box

The gene

Basic information

• Locus tag: BSU13230

Database entries

• BsubCyc: BSU13230

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • ThiW-(ThiV-ThiX)-ThiU PubMed

• Localization: membrane associated PubMed

Database entries

• BsubCyc: BSU13230

• Structure:

• UniProt: O34738

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: thiU-thiV-thiW-thiX PubMed

• Expression browser: thiV PubMed

• Sigma factor:

• Regulation:
  • repressed by thiamine (Thi-box) PubMed

• Regulatory mechanism:
  • Thi-box: RNA switch PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 360 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Ghislain Schyns, Sébastien Potot, Yi Geng, Teresa M Barbosa, Adriano Henriques, John B Perkins
Isolation and characterization of new thiamine-deregulated mutants of Bacillus subtilis.
J Bacteriol: 2005, 187(23);8127-36
[PubMed:16291685] [WorldCat.org] [DOI] (P p)

Dmitry A Rodionov, Alexey G Vitreschak, Andrey A Mironov, Mikhail S Gelfand
Comparative genomics of thiamin biosynthesis in procaryotes. New genes and regulatory mechanisms.
J Biol Chem: 2002, 277(50);48949-59
[PubMed:12376536] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)