yhdT

yhdT
168

similar to magnesium exporter

Locus
BSU_09590
Molecular weight
51.35 kDa
Isoelectric point
4.75
Protein length
461 aaSequenceBlast
Gene length
1386 bpSequenceBlast
Function
unknown
Product
unknown
Essential
no
Synonyms
yhdT
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1253 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,035,554 → 1,036,939
The protein
Protein family
UPF0053 family (according to UniProt)
Domains
CNNM transmembrane domain (aa 1-202) (according to UniProt)
DUF21 domain (7-202)
2 CBS domains (aa 221-280, aa 290-347) (according to UniProt)
Structure
4HG0 (PDB) (CorC from E. coli, corresponds to the CBS domains and the C-terminal transporter-associated domain)
O07589 (AlphaFold)
Paralogous protein(s)
MpfA, YqhB, YrkA, YugS
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
yhdT
Description
PubMed
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yhdT

2025-03-15 08:44:22

Jstuelk

81

9fc3909755d86d16768d8c922560d340f21e36a5

11325275FFD543252FC6389DA31A8671202407DB

Biological materials
Mutant
MGNA-A698 (yhdT::erm), available at the NBRP B. subtilis, Japan
BKE09590 (ΔyhdT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTAACCCTATCCTCTCA,  downstream forward: _UP4_AGCGAGAAATAAGCTCAAGG
BKK09590 (ΔyhdT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTAACCCTATCCTCTCA,  downstream forward: _UP4_AGCGAGAAATAAGCTCAAGG
Expression vectors
pGP2928 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
pGP3464 (215-351aa) (N-terminal 6xHis-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
pGP3469 (215-351aa) (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in Jörg Stülke's lab
References
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Page visits: 4693

Time of last update: 2025-03-31 00:43:55

Author of last update: Jstuelk