rlmCD

rlmCD
168

rRNA methyltransferase

Locus
BSU_06730
Molecular weight
51.65 kDa
Isoelectric point
5.71
Protein length
459 aaSequenceBlast
Gene length
1380 bpSequenceBlast
Function
23S rRNA maturation
Product
rRNA methyltransferase
Essential
no
E.C.
2.1.1.190
Synonyms
rlmCD, yerS, yefA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2265 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
737,603 → 738,982
The protein
Catalyzed reaction/ biological activity
methylation of U747 and U1939 in 23S rRNA PubMed
S-adenosyl-L-methionine + uridine747 in 23S rRNA --> 5-methyluridine747 in 23S rRNA + H+ + S-adenosyl-L-homocysteine (according to UniProt)
S-adenosyl-L-methionine + uridine1939 in 23S rRNA --> 5-methyluridine1939 in 23S rRNA + H+ + S-adenosyl-L-homocysteine (according to UniProt)
Protein family
Methyltransferase superfamily (according to UniProt)
class I-like SAM-binding methyltransferase superfamily (according to UniProt)
Domains
TRAM domain (aa 6-64) (according to UniProt)
Cofactors
Fe-S cluster PubMed
Structure
2BH2 (PDB) (from E. coli, 32% identity) PubMed
O31503 (AlphaFold)
Paralogous protein(s)
YfjO
Expression and Regulation
Operons
Genes
dgkB-rlmCD
Description
PubMed
Open in new tab

dgkB→rlmCD

2025-04-06 22:58:17

Jstuelk

94

c4890a496ded0653f7505864ebeaa248fc91af7f

322B8BEE164A821FAFE0FA8F102DB8F2A1E931B4

Genes
dgkB-rlmCD-yefC-dnmA-yeeB-yeeC
Description
PubMed
Open in new tab

dgkB→yeeC

2025-03-14 08:31:08

Jstuelk

126

ff916330f6b5023fdc317a5fb342282b6227f373

336CDC790AAC5927B58A940F2B9646F19FD7FCCD

Biological materials
Mutant
MGNA-A931 (yefA::erm), available at the NBRP B. subtilis, Japan
BKE06730 (ΔrlmCD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTATAATCACCTCTAGT,  downstream forward: _UP4_TTAAAAGAATAAATAGTCAA
BKK06730 (ΔrlmCD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTATAATCACCTCTAGT,  downstream forward: _UP4_TTAAAAGAATAAATAGTCAA
References
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B97ABE3233A27A7C8273ED9A1E688247DD37FD12

Page visits: 2303

Time of last update: 2025-04-08 01:07:37

Author of last update: Melvin.boenninger