tsaE

tsaE
168

protein kinase, required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA

Locus
BSU_05910
Molecular weight
17.76 kDa
Isoelectric point
4.6
Protein length
158 aaSequenceBlast
Gene length
477 bpSequenceBlast
Function
control of tRNA modification
Product
protein kinase involved in tRNA modification
Essential
no
Synonyms
tsaE, ydiB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0802 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
641,654 → 642,130
Phenotypes of a mutant
reduced growth rate  PubMed
increased sensitivity to paraquat PubMed
The protein
Catalyzed reaction/ biological activity
biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP (TsaB-TsaD-TsaE) PubMed
autophosphorylation on Ser-60, Thr-55, Thr-62, and Thr-64 PubMed
phosphorylation of TsaD PubMed
Protein family
TsaE family (single member, according to UniProt)
Cofactors
Mg2+ PubMed
Structure
5MVR (PDB) PubMed
O05515 (AlphaFold)
Modification
autophosphorylation on Ser-60, Thr-55, Thr-62, and Thr-64 PubMed
Kinetic information
K(M) for ATP: 60 myM, V(max) 10 nmol/min  PubMed
Localization
cytoplasm with the exception of the nucleoid region  PubMed
Additional information
ATPase activity is required for in vivo function of the protein  PubMed
Expression and Regulation
Operons
Genes
thiL-tsaE-tsaB-rimI-tsaD
Description
PubMed
Open in new tab

thiL→tsaD

2025-03-29 12:30:01

ghost

126

45839886739c513a86015eb2db5091e043ecd558

F160E3DB3BAB4687DBF8F609242CE813EE9E1BF6

Biological materials
Mutant
BKE05910 (ΔtsaE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTGCTTCACACGAGTCACCC,  downstream forward: _UP4_ATGCTTTGTGAGGAGTTAAG
BKK05910 (ΔtsaE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTGCTTCACACGAGTCACCC,  downstream forward: _UP4_ATGCTTTGTGAGGAGTTAAG
Expression vectors
for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP833, available in Stülke lab
for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP841, available in Stülke lab
References
Loading

E15BB518649B2B9CD44CEBBB4FA481E55BCE823C

Page visits: 5156

Time of last update: 2025-03-31 21:51:27

Author of last update: Jstuelk