skfA

skfA
168

spore killing factor

Locus
BSU_01910
Molecular weight
5.80 kDa
Isoelectric point
10.65
Protein length
55 aaSequenceBlast
Gene length
168 bpSequenceBlast
Function
killing of sister cells
Product
spore killing factor
Essential
no
Synonyms
skfA, ybcO
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms

This gene is a member of the following regulons

Gene
Coordinates
213,941 → 214,108
The protein
Structure
O31422 (AlphaFold)
Modification
the mature SKF is cyclic 26-amino acid peptide that is posttranslationally modified with one disulfide and one cysteine thioether bridged to the alpha-position of a methionine PubMed, the thioether bond is generated by SkfB PubMed
Localization
secreted (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
skfA-skfB-skfC-skfE-skfF-skfG-skfH
Description
PubMed
Regulation
expressed under conditions that trigger sporulation (Spo0A) PubMed
Regulatory mechanism
Spo0A: activation, PubMed, in spo0A regulon
AbrB: repression, PubMed, in abrB regulon
PhoP: activation, PubMed, in phoP regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
Northern blotting during during phosphate limitation showed an intense 0.25 kb 'skfA'-specific transcript, and a weaker 6.5 kb 'skfA-skfB-skfC-skfE-skfF-skfG-skfH' transcript.
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skfA→skfH

2025-03-31 01:14:49

ghost

166

f96cb932f6aa46632dc613334e3526e3904b2534

E1A5B1DE7F5A4653FA0FC2E7FE7AAD3454DBFF28

Additional information
the mRNA is very stable (> 15 min) PubMed
Biological materials
Mutant
MGNA-B971 (ybcO::erm), available at the NBRP B. subtilis, Japan
BKE01910 (ΔskfA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAGTAAACCTCCTCTCA,  downstream forward: _UP4_TAACATTTGAGAATAGGGAG
BKK01910 (ΔskfA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAGTAAACCTCCTCTCA,  downstream forward: _UP4_TAACATTTGAGAATAGGGAG
GFP fusion
GP1645 ''ymdB::spc Pskf-gfp (cat'') in the NCIB3610 background, available in Jörg Stülke's lab
References
Reviews
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Original Publications
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DDB0022F50999AC52809D651C9CC5A5FDC71302C

Page visits: 6946

Time of last update: 2025-04-03 09:33:08

Author of last update: Jstuelk