pucG

pucG
168

(S)-ureidoglycine-glyoxylate aminotransferase

Locus
BSU_32520
Molecular weight
45.58 kDa
Isoelectric point
5.42
Protein length
416 aaSequenceBlast
Gene length
1251 bpSequenceBlast
Function
purine utilization
Product
(S)-ureidoglycine-glyoxylate aminotransferase
Essential
no
Synonyms
pucG, yurG
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0075 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,341,166  3,342,416
The protein
Catalyzed reaction/ biological activity
transamination between an unstable intermediate ((S)-ureidoglycine) and the end product of purine catabolism (glyoxylate) to yield oxalurate and glycine PubMed
(S)-2-ureidoglycine + glyoxylate --> glycine + N-carbamoyl-2-oxoglycine (according to UniProt)
Protein family
Class-V pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)
Cofactors
PLP
Structure
3ISL (PDB)\tPubMed
O32148 (AlphaFold)
Expression and Regulation
Operons
Genes
pucF-pucG
Description
PubMed
Regulation
induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed
Expression
expression is heterogeneous PubMed
Regulatory mechanism
PucR: activation, PubMed, in pucR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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pucF→pucG

2025-03-15 06:39:16

Jstuelk

101

8950B371B49B2D867C39C0645E288618F9712782

C7BE4A3C8762E1DBFB97C41D65DC9339DE1A1137

Biological materials
Mutant
MGNA-A942 (yurG::erm), available at the NBRP B. subtilis, Japan
BKE32520 (pucG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCCTGACACAGCCATTCCTC,  downstream forward: _UP4_TAAAGAAAAGCTTGCGGAAC
BKK32520 (pucG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCCTGACACAGCCATTCCTC,  downstream forward: _UP4_TAAAGAAAAGCTTGCGGAAC
References
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4BC4B3E0AB4779D1DD5EDF591361FC1094AF661F

Page visits: 3505

Time of last update: 2025-04-01 10:18:59

Author of last update: Melvin.boenninger