pstBA

pstBA
168

phosphate ABC transporter (ATP-binding protein)

Locus
BSU_24960
Molecular weight
29.85 kDa
Isoelectric point
7.9
Protein length
269 aaSequenceBlast
Gene length
810 bpSequenceBlast
Function
high-affinity phosphate uptake
Product
phosphate ABC transporter (ATP-binding protein)
Essential
no
E.C.
3.6.3.27
Synonyms
pstBA, pstB, yqgJ, yzmE
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1117 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,578,003  2,578,812
Additional information
the pstBA gene is often inactivated during adaptation to Arabidopsis thaliana PubMed
The protein
Catalyzed reaction/ biological activity
ATP + H2O + phosphate --> ADP + H+ + 2 phosphate (according to UniProt)
Protein family
ABC transporter superfamily (according to UniProt)
Domains
ABC transporter domain (aa 23-264) (according to UniProt)
Structure
2OLJ (PDB) (from Geobacillus stearothermophilus, 37% identity)
P46341 (AlphaFold)
Paralogous protein(s)
GlnQ, ArtR, TcyN, TcyC, YxeO, PstBB
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
pstS-pstC-pstA-pstBA-pstBB
Description
PubMed
Regulation
expressed under conditions of phosphate limitation (5000-fold induced) (PhoP) PubMed
Regulatory mechanism
PhoP: activation, PhoP-P, PubMed, in phoP regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
mRNA processing between pstS and pstC to maintain higher concentrations of PstS relative to other components of the ABC transporter PubMed
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pstS→pstBB

2025-04-10 04:00:01

Bzhu

121

8049186c5321c6357daaaa636ef07838c5d49959

317615DB0F936F19331B43F8E7921710E57393F5

Biological materials
Mutant
MGNA-C440 (yqgJ::erm), available at the NBRP B. subtilis, Japan
BKE24960 (pstBA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTTTCGCCTCTTTCC,  downstream forward: _UP4_GGGAAATTCGGATAGGAGGG
BKK24960 (pstBA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTTTCGCCTCTTTCC,  downstream forward: _UP4_GGGAAATTCGGATAGGAGGG
Labs working on this gene/protein
Marion Hulett, University of Illinois at Chicago, USA Homepage
References
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434BCED6953BE07D592AC229264C5AD0D04CBAF6

Page visits: 4497

Time of last update: 2025-04-17 17:02:26

Author of last update: Jstuelk