yqeV
168

tRNA methylthiotransferase

Locus

BSU_25430

Molecular weight

51.49 kDa

Isoelectric point

5.24

Protein length

451 aaSequenceBlast

Gene length

1356 bpSequenceBlast

Function

tRNA maturation

Product

tRNA methylthiotransferase

Essential

Synonyms

mtaB, yqeV

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0621 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

2,621,677 2,623,032

The protein

Catalyzed reaction/ biological activity

modification of N(6)-threonylcarbamoyladenosine (t(6)A) to 2-methylthio-N(6)-threonylcarbamoyladenosine (ms(2)t(6)A) in tRNA PubMed

[sulfur carrier]-SH + AH₂ + N⁶-L-threonylcarbamoyladenosine³⁷ in tRNA + 2 S-adenosyl-L-methionine --> 2-methylsulfanyl-N⁶-L-threonylcarbamoyladenosine³⁷ in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H⁺ + L-methionine + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

methylthiotransferase family (with YmcB, according to UniProt)

Domains

MTTase N-terminal domain (aa 2-114) (according to UniProt)

TRAM domain (aa 372-437) (according to UniProt)

Cofactors

2 Fe-S clusters PubMed

Structure

4JC0 (PDB) (from Thermotoga maritima, 29% identity) PubMed

P54462 (AlphaFold)

Paralogous protein(s)

YmcB