melA

melA
168

alpha-galactosidase

Locus
BSU_30300
Molecular weight
49.10 kDa
Isoelectric point
5.84
Protein length
432 aaSequenceBlast
Gene length
1299 bpSequenceBlast
Function
utilization of melibiose and raffinose family oligosaccharides (raffinose, stachyose)
Product
alpha-galactosidase
Essential
no
E.C.
3.2.1.22
Synonyms
melA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1486 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,100,881 → 3,102,179
The protein
Catalyzed reaction/ biological activity
Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids (according to UniProt)
Protein family
Glycosyl hydrolase 4 family (according to UniProt)
Cofactors
NAD+ PubMed
Mn2+ PubMed
Structure
5C3M (PDB) (from Geobacillus stearothermophilus, 28% identity)
O34645 (AlphaFold)
Kinetic information
KM for melibiose: 10 mM PubMed
KM for raffinose: 25 mM PubMed
Expression and Regulation
Operons
Genes
melA
Description
PubMed
Regulation
repressed by glucose (CcpA) PubMed
Open in new tab

melA

2025-03-23 10:25:20

ghost

85

1bc7cb4d9e2672f66c86d9957ba581d6a8fde7dc

5E4752D30FD4B6FE2919B2FB6D5F9D151C765073

Genes
melE-melD-melC-melA
Description
PubMed
Regulation
repressed by glucose (CcpA) PubMed
Open in new tab

melE→melA

2025-03-30 22:32:13

ghost

90

847594f17f75304119fc4e01c75357cb3be0099d

6C880CEB4C8A523152A9E2F2B771FB0BEA14B117

Genes
melR-melE-melD-melC-melA
Description
PubMed
Regulation
repressed by glucose (CcpA) PubMed
Regulatory mechanism
CcpA: repression, PubMed, in ccpA regulon
MelR: repression, PubMed, in melR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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melR→melA

2025-03-16 15:58:26

ghost

90

a76a3bd8bbf487172d83c078a68b2450f4f95213

AB0F662445BB81BBCFC1971F2E98536D4BBE6D03

Biological materials
Mutant
BKE30300 (ΔmelA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCATATTGCTTCCCCCTT,  downstream forward: _UP4_TAAAAACTAGGGGACCGCTC
BKK30300 (ΔmelA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCATATTGCTTCCCCCTT,  downstream forward: _UP4_TAAAAACTAGGGGACCGCTC
References
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CDE9EF9CFB799794D525F12DEC8C4190C6298575

Page visits: 3773

Time of last update: 2025-04-01 03:21:23

Author of last update: Melvin.boenninger