tcyN

tcyN
168

cystine and cysteinopantetheine ABC transporter (ATP-binding protein)

Locus
BSU_29340
Molecular weight
29.41 kDa
Isoelectric point
8.84
Protein length
259 aaSequenceBlast
Gene length
780 bpSequenceBlast
Function
cystine and cysteinopantetheine uptake
Product
cystine and cysteinopantetheine ABC transporter (ATP-binding protein)
Essential
no
Synonyms
tcyN, hisP, ytmN
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG1126 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,004,346 → 3,005,125
The protein
Catalyzed reaction/ biological activity
hydrolysis of ATP for the uptake of cystine and cysteinopantetheine PubMed
Protein family
ABC transporter superfamily (according to UniProt)
Domains
ABC transporter domain (aa 2-239) (according to UniProt)
Structure
2Q0H (PDB) (complex with ADP-Mg2+, Geobacillus stearothermophilus, 54%  identity )
O34900 (AlphaFold)
Paralogous protein(s)
TcyC, YxeO, PstBB, YvrO, GlnQ, ArtR, PstBA
Localization
attached to the membrane via TcyL-TcyM PubMed
Expression and Regulation
Operons
Genes
snaA-tcyJ-tcyK-tcyL-tcyM-tcyN-cmoO-cmoI-cmoJ-ribR-sndA-ytnM
Description
PubMed
Regulation
induced in the presence of methionine and taurine PubMed
Regulatory mechanism
CymR: repression, PubMed, in cymR regulon
AscR: activation, PubMed, in ascR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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snaA→ytnM

2025-03-22 23:22:48

Jstuelk

115

8a3868e633778d2f68bf47d615f22f716e843294

4D6CA410A1DC104A491D3C18248CCD50CC0E983A

Biological materials
Mutant
GP4468 (trpC2 ΔtcyN::neo), available in Jörg Stülke's lab
GP4676 (trpC2 ΔtcyN::lox72), available in Jörg Stülke's lab
GP4659 (trpC2 ΔtcyJ-tcyK-tcyL-tcyM-tcyN::tet), available in Jörg Stülke's lab PubMed
BKE29340 (ΔtcyN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTATGGATATTTTTAATCT,  downstream forward: _UP4_GAACACATAAAGGAGCCGGT
BKK29340 (ΔtcyN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTATGGATATTTTTAATCT,  downstream forward: _UP4_GAACACATAAAGGAGCCGGT
Expression system
GP4687 (trpC2 xkdE::Pxyl-tcyJ-tcyK-tcyL-tcyM-tcyN-ermC), available in Jörg Stülke's lab
References
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Time of last update: 2025-04-02 20:30:51

Author of last update: Jstuelk