fbaA

fbaA
168

fructose-1,6-bisphosphate aldolase, glycolytic/ gluconeogenic enzyme

Locus
BSU_37120
Molecular weight
30.25 kDa
Isoelectric point
5.03
Protein length
285 aaSequenceBlast
Gene length
858 bpSequenceBlast
Function
enzyme in glycolysis/ gluconeogenesis
Product
fructose-1,6-bisphosphate aldolase
Essential
no
E.C.
4.1.2.13
Synonyms
fbaA, fba, fba1, tsr
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0191 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,808,512 3,809,369
Phenotypes of a mutant
essential PubMed, non-essential according to PubMed
The protein
Catalyzed reaction/ biological activity
D-fructose-1,6-bisphosphate --> D-glyceraldehyde-3-P + dihydroxyacetone-P (according to UniProt)
Protein family
class II fructose-bisphosphate aldolase family (with IolJ, according to UniProt)
Domains
2 x dihydroxyacetone-P binding domain (210212), (231234)
Cofactors
Zn2+ (Metalloenzyme)
Structure
3Q94 (PDB) (from Bacillus anthracis)
P13243 (AlphaFold)
Modification
phosphorylation on Thr-212 and Thr-234 PubMed
Effectors of protein activity
Inhibited by 2-oxoglutarate, oxaloacetate and pyruvate PubMed PubMed
Activated by NH4+ PubMed
Paralogous protein(s)
IolJ
Additional information
Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution
extensive information on the structure and enzymatic properties of FbaA can be found at Proteopedia
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
fbaA
Description
PubMed
Regulation
constitutively expressed PubMed
Open in new tab

fbaA

2025-03-22 22:47:08

ghost

80

b23bd4d20860aa2cbd12c1df4a5b0dbe0987693a

794BD730BB0613B1D4766C3206D0909D47CD7322

Genes
spo0F-fbaA-ywjH-murAB-glpX-rho-rpmE
Description
PubMed
Expression
readthrough-transcription from the SigH-dependent promoter after the initiation of sporulation in the mother cell PubMed
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spo0F→rpmE

2025-03-16 05:53:33

Jstuelk

27

C63B272CC9442316212569FF9E0451D7AD583B70

C10FBD1184EE96831E587248498E99020C63607C

Genes
fbaA-ywjH
Description
PubMed
Regulation
constitutively expressed PubMed
Open in new tab

fbaA→ywjH

2025-04-01 20:23:49

ghost

116

3de4c158d0f59e42a135e3344384ecf580467739

F733D4A7CF3474C9704F8FE6039F28B670F0B03B

Biological materials
Mutant
GP591 (fbaA::cat), available in Jrg Stlke's lab, PubMed
GP596 (fbaA::erm), available in Jrg Stlke's lab, PubMed
BKE37120 (fbaA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCGAATGTCCTCCTTA, downstream forward: _UP4_TAATTCAATTGGAACTTTTT
BKK37120 (fbaA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCGAATGTCCTCCTTA, downstream forward: _UP4_TAATTCAATTGGAACTTTTT
Expression vectors
for expression in B. subtilis, in pBQ200: pGP1423, available in Jrg Stlke's lab
for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP88, available in Jrg Stlke's lab, PubMed
for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP395, available in Jrg Stlke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jrg Stlke's lab PubMed
LacZ fusion
pGP601 (in pAC6), available in Jrg Stlke's lab PubMed
References
Reviews
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Original Publications
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800EC1F1CA8C4F2A643EDECBB684347C29CAB0CA

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Time of last update: 2025-04-03 00:46:27

Author of last update: Jstuelk