degV

degV
168

fatty acid binding protein

Locus
BSU_35480
Molecular weight
31.43 kDa
Isoelectric point
5.23
Protein length
281 aaSequenceBlast
Gene length
846 bpSequenceBlast
Function
phosphorylation of fatty acids
Product
fatty acid binding protein
Essential
no
E.C.
3.6.1.1
Synonyms
degV, yviA, sacU
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1307 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,643,664 → 3,644,509
The protein
Catalyzed reaction/ biological activity
binds and presents fatty acids for phosphorylation to FakA (based on findings for homologous proteins from Staphylococcus aureus) PubMed
Domains
DegV domain (aa 3-280) (according to UniProt)
Structure
3FYS (PDB) PubMed
P32436 (AlphaFold)
Paralogous protein(s)
YitS
Expression and Regulation
Operons
Genes
degV
Description
PubMed
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degV

2025-03-16 12:28:24

ghost

77

b8006734dba7d27a40c0b4be5602a485f310af6e

64693F93C962250609D46F4A550AEDCE5031C4C2

Biological materials
Mutant
GP3757 (ΔdegV::spc), available in Jörg Stülke's lab
MGNA-A387 (yviA::erm), available at the NBRP B. subtilis, Japan
BKE35480 (ΔdegV::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGCTTCGATACCTGCCT,  downstream forward: _UP4_TAAGGCCAAATCTCCGTTTT
BKK35480 (ΔdegV::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGCTTCGATACCTGCCT,  downstream forward: _UP4_TAAGGCCAAATCTCCGTTTT
Expression vectors
pGP3627 (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in Jörg Stülke's lab
References
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Time of last update: 2025-04-01 09:11:47

Author of last update: Dennis.wicke