cspD

cspD
168

cold shock protein

Locus
BSU_21930
Molecular weight
7.17 kDa
Isoelectric point
4.31
Protein length
66 aaSequenceBlast
Gene length
201 bpSequenceBlast
Function
RNA chaperone
Product
cold shock protein
Essential
no
Synonyms
cspD
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1278 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,307,905 2,308,105
Phenotypes of a mutant
cspB cspD double mutants are poorly viable, rapidly acquire suppressor mutations that result in overexpression of cspC or loss of the veg gene PubMed
cspB cspD double mutants have lost genetic competence PubMed
The protein
Domains
CSD domain (aa 4-63) (according to UniProt)
Structure
1C9O (PDB) (from B. caldolyticus, 86% identity) PubMed
P51777 (AlphaFold)
Paralogous protein(s)
CspB, CspC
Additional information
subject to Clp-dependent proteolysis upon glucose starvation PubMed
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
cspD
Description
PubMed
Expression
strong constitutive expression PubMed
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cspD

2025-03-17 20:06:30

Jstuelk

105

CE5C78259E09AA3AD33DD6FA66E5DCB0E85DCB5B

63D0853793A9B47C57432809A3B339EB393E3B45

Biological materials
Mutant
GP2614 (cspD::aphA3), available in Jörg Stülke's lab PubMed
MGNA-A874 (cspD::erm), available at the NBRP B. subtilis, Japan
BKE21930 (cspD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTGCTTAATTCCTCCT, downstream forward: _UP4_TAAACTCAATACATGATGAT
BKK21930 (cspD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTGCTTAATTCCTCCT, downstream forward: _UP4_TAAACTCAATACATGATGAT
Expression vectors
pGP2164: expression of cspD-Strep by pGP382 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
pGP2165: expression of Strep-cspD by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
LacZ fusion
GP3286 (cspD::lacZ cat), available in Jörg Stülke's lab PubMed
References
Reviews
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Original Publications
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Time of last update: 2025-04-02 10:52:42

Author of last update: Jstuelk