pdxT
168

pyridoxal-5-phosphate synthase (glutaminase domain)

Locus

BSU_00120

Molecular weight

21.30 kDa

Isoelectric point

4.98

Protein length

196 aaSequenceBlast

Gene length

591 bpSequenceBlast

Function

pyridoxal-5-phosphate biosynthesis

Product

pyridoxal-5-phosphate synthase (glutaminase domain)

Essential

E.C.

4.3.3.6

Synonyms

pdxT, yaaE

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0311 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

19,968 20,558

Phenotypes of a mutant

the mutant tends to acquire suppressor mutations that result in improved growth and colony morphology PubMed

auxotrophic for pyridoxal-5-phosphate at low external ammonium concentration PubMed, this can be suppressed by overexpression of pdxS or the ammonium transporter NrgA PubMed

inactivation of pdxT reduces sporulation efficiency to 1.2% that of wild type cells; delayed entry into sporulation PubMed

poor growth PubMed

non-transformable PubMed

The protein

Catalyzed reaction/ biological activity

Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS. (according to UniProt)

aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine --> H⁺ + 3 H₂O + L-glutamate + phosphate + pyridoxal 5'-phosphate (according to UniProt)

Protein family

Glutaminase PdxT/SNO family (single member, according to UniProt)

Structure

2NV2 (PDB) PdxS-PdxT complex PubMed

2NV0 (PDB) (PdxT) PubMed

P37528 (AlphaFold)

Localization

cytosol (according to UniProt)

Expression and Regulation

Operons

Genes

dacA-pdxS-pdxT-serS