acuB

acuB
168

unknown

Locus
BSU_29700
Molecular weight
24.21 kDa
Isoelectric point
6.59
Protein length
214 aaSequenceBlast
Gene length
645 bpSequenceBlast
Function
unknown
Product
unknown
Essential
no
Synonyms
acuB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG3448 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, CcpA regulon

Gene
Coordinates
3,040,751 → 3,041,395
The protein
Domains
2 CBS domains (aa 7-66, aa 78-135) (according to UniProt)
Structure
5AWE (PDB) (from Thermus thermophilus, 30% identity) PubMed
P39066 (AlphaFold)
Localization
Cytoplasm (Homogeneous) PubMed
Expression and Regulation
Operons
Genes
acuA-acuB-acuC
Description
PubMed
Regulation
repressed by glucose (3.4-fold) (CcpA) PubMed
Regulatory mechanism
CcpA: repression, PubMed, in ccpA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
the mRNA is quite stable (half-life 5 min) PubMed
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acuA→acuC

2025-03-09 17:45:53

Jstuelk

121

0c83d2dce6fb2fd652b90f3db87e253e0dd5a2cb

C4FEF5C006237E0BF05F8B8DB65202D8D51016FB

Biological materials
Mutant
BKE29700 (ΔacuB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTATTTTTTCCCCTTTGT,  downstream forward: _UP4_CCGTCAGAGCAAAGGGATCT
BKK29700 (ΔacuB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTATTTTTTCCCCTTTGT,  downstream forward: _UP4_CCGTCAGAGCAAAGGGATCT
Expression vectors
pGP2922 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
References
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Page visits: 4363

Time of last update: 2025-04-01 19:24:20

Author of last update: Melvin.boenninger