epsN

epsN
168

UDP-2,6-dideoxy 2-acetamido 4-keto glucose aminotransferase, required for extracellular polysaccharide synthesis

Locus
BSU_34230
Molecular weight
42.76 kDa
Isoelectric point
5.84
Protein length
388 aaSequenceBlast
Gene length
1167 bpSequenceBlast
Function
Biofilm formation, biosynthesis of UDP-N,N’-diacetylbacillosamine (with EpsC and EpsM)
Product
UDP-2,6-dideoxy 2-acetamido 4-keto glucose aminotransferase
Essential
no
Synonyms
epsN, yvfE
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0399 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,515,062  3,516,228
Phenotypes of a mutant
smooth colonies on MsGG medium, no biofilm formation PubMed
The protein
Catalyzed reaction/ biological activity
UDP-2,6-dideoxy 2-acetamido 4-keto glucose + glutamate --> UDP-2,6-dideoxy 2-acetamido 4-amino glucose + 2-oxoglutarate PubMed
Protein family
DegT/DnrJ/EryC1 family (with NtdA and SpsC, according to UniProt)
Cofactors
pyridoxal phosphate PubMed
Structure
1O61 (PDB) (from Campylobacter jejuni, 46% identity) 16021622
Q795J3 (AlphaFold)
Expression and Regulation
Operons
Genes
epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO
Description
PubMed
Regulation
repressed by SinR PubMed
Regulatory mechanism
RemA: activation, PubMed, in remA regulon
AbrB: repression, PubMed, in abrB regulon
SinR: anti-activation, (prevents binding of RemA) PubMed, in sinR regulon
EAR riboswitch: processive antitermination, in EAR riboswitch
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
induction by sequestration of SinR by SinI or SlrA PubMed
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epsA→epsO

2025-04-02 11:08:39

ghost

150

972f8e32dbf02ccc05ec1b86b52f3e7c715bf4da

DAC70A15EA1F43CB18727990D26DF72F59759165

Biological materials
Mutant
MGNA-A064 (yvfE::erm), available at the NBRP B. subtilis, Japan
BKE34230 (epsN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAAGTAGATTTTTTTATGCA,  downstream forward: _UP4_TTCCATACTGTCGAGGTGAA
BKK34230 (epsN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAAGTAGATTTTTTTATGCA,  downstream forward: _UP4_TTCCATACTGTCGAGGTGAA
Labs working on this gene/protein
Richard Losick, Harvard Univ., Cambridge, USA homepage
References
Reviews
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Research papers
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The EAR RNA switch
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0D24AB8D446CFFBC6DEAD3C10D1FA59551545FE8

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Time of last update: 2025-04-17 05:42:50

Author of last update: Jstuelk