ykkE

ykkE
168

formyltetrahydrofolate deformylase

Locus
BSU_13110
Molecular weight
34.34 kDa
Isoelectric point
5.95
Protein length
300 aaSequenceBlast
Gene length
903 bpSequenceBlast
Function
purine nucleotide synthesis
Product
formyltetrahydrofolate deformylase
Essential
no
E.C.
3.5.1.10
Synonyms
ykkE, purU
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0788 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,377,243  1,378,145
The protein
Catalyzed reaction/ biological activity
(6S)-10-formyltetrahydrofolate + H2O --> (6S)-5,6,7,8-tetrahydrofolate + formate + H+ (according to UniProt)
Protein family
PurU family (single member, according to UniProt)
Domains
ACT domain (aa 21-102) (according to UniProt)
Structure
3W7B (PDB) (from Thermus thermophilus, 58% identity) PubMed
O34990 (AlphaFold)
Paralogous protein(s)
PurN
Expression and Regulation
Operons
Genes
ykkE
Description
PubMed
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ykkE

2025-03-17 19:19:33

ghost

66

834e5a9df21949203b959e9d3a180ba892a2d22f

FCE88EF8CE5E05C009FE8080EE315AF3228F840C

Biological materials
Mutant
MGNA-B310 (ykkE::erm), available at the NBRP B. subtilis, Japan
BKE13110 (ykkE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAATAATCCCTCTTATC,  downstream forward: _UP4_TAGACTGCAAGAGGCCCGCG
BKK13110 (ykkE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAATAATCCCTCTTATC,  downstream forward: _UP4_TAGACTGCAAGAGGCCCGCG
References
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Time of last update: 2025-04-03 02:43:58

Author of last update: Jstuelk