FosB

• Description: bacillithiol-S-transferase; confers resistence against antimicrobial compounds from B. amyloliquefaciens
  
  

Gene name	fosB
Synonyms	yndN
Essential	no
Product	fosfomycin resistance protein
Function	confers resistence against antimicrobial compounds from B. amyloliquefaciens
Gene expression levels in SubtiExpress: fosB
MW, pI	17 kDa, 6.137
Gene length, protein length	432 bp, 144 aa
Immediate neighbours	yndM, lexA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell envelope stress proteins (controlled by SigM, V, W, X, Y), resistance against toxins/ antibiotics

This gene is a member of the following regulons

SigW regulon

The gene

Basic information

• Locus tag: BSU17840

Database entries

• BsubCyc: BSU17840

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: FosB subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU17840

• Structure:

• UniProt: O31817

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: fosB PubMed

• Expression browser: fosB PubMed

• Sigma factor: SigW PubMed

• Regulation:
  • expressed under stress conditions (SigW) PubMed

• Regulatory mechanism:

• Additional information:
  • A ncRNA is predicted between fosB and lexA PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Alexandra A Roberts, Sunil V Sharma, Andrew W Strankman, Shayla R Duran, Mamta Rawat, Chris J Hamilton
Mechanistic studies of FosB: a divalent-metal-dependent bacillithiol-S-transferase that mediates fosfomycin resistance in Staphylococcus aureus.
Biochem J: 2013, 451(1);69-79
[PubMed:23256780] [WorldCat.org] [DOI] (I p)

Alexander P Lamers, Mary E Keithly, Kwangho Kim, Paul D Cook, Donald F Stec, Kelly M Hines, Gary A Sulikowski, Richard N Armstrong
Synthesis of bacillithiol and the catalytic selectivity of FosB-type fosfomycin resistance proteins.
Org Lett: 2012, 14(20);5207-9
[PubMed:23030527] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Bronwyn G Butcher, John D Helmann
Identification of Bacillus subtilis sigma-dependent genes that provide intrinsic resistance to antimicrobial compounds produced by Bacilli.
Mol Microbiol: 2006, 60(3);765-82
[PubMed:16629676] [WorldCat.org] [DOI] (P p)

M Cao, B A Bernat, Z Wang, R N Armstrong, J D Helmann
FosB, a cysteine-dependent fosfomycin resistance protein under the control of sigma(W), an extracytoplasmic-function sigma factor in Bacillus subtilis.
J Bacteriol: 2001, 183(7);2380-3
[PubMed:11244082] [WorldCat.org] [DOI] (P p)

23256780