nasD

nasD
168

assimilatory nitrite reductase (subunit)

Locus
BSU_03300
Molecular weight
88.24 kDa
Isoelectric point
4.95
Protein length
805 aaSequenceBlast
Gene length
2418 bpSequenceBlast
Function
utilization of nitrite as nitrogen source
Product
assimilatory nitrite reductase (subunit)
Essential
no
E.C.
1.7.1.4
Synonyms
nasD, nasBC, nirB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1251 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
355,764 → 358,181
The protein
Catalyzed reaction/ biological activity
2 H2O + 3 NADP+ + NH4+ --> 5 H+ + 3 NADPH + nitrite (according to UniProt)
2 H2O + 3 NAD+ + NH4+ --> 5 H+ + 3 NADH + nitrite (according to UniProt)
Protein family
nitrite and sulfite reductase 4Fe-4S domain family (with CysI, according to UniProt)
Cofactors
Fe-S cluster  PubMed
FAD  PubMed
Structure
3KLJ (PDB) (from Clostridium acetobutylicum, corresponds to aa 5 ... 399, 32% identity) PubMed
P42435 (AlphaFold)
Paralogous protein(s)
NasB
Expression and Regulation
Operons
Genes
nasB-nasC-nasD-nasE-nasF
Description
PubMed
Regulation
''nasD'': expressed under anaerobic conditions (ResD) PubMed
Regulatory mechanism
ResD: activation, PubMed, in resD regulon
TnrA: activation, PubMed, in tnrA regulon
NsrR: repression, PubMed, in nsrR regulon
Fur: repression, PubMed, in fur regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

nasB→nasF

2025-03-28 12:14:50

ghost

125

f7e39891ce616efe86a59a87e70448f347bb9239

B3FDABF6DE6A38B053CBE30BA36088850B35E312

Genes
nasD-nasE-nasF
Description
PubMed
Regulation
''nasD'': expressed under anaerobic conditions (ResD) PubMed
Open in new tab

nasD→nasF

2025-03-17 09:49:09

ghost

103

5ad87c79ecdfd1b1041c676834950d8393689a20

E10C8C0393696A6DF68E202566914889ADC48A5C

Biological materials
Mutant
1A973 ( nasD::phleo), PubMed, available at BGSC
BKE03300 (ΔnasD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAGATGATCCGCTCCTT,  downstream forward: _UP4_TAATGACAAAAACTATCATT
BKK03300 (ΔnasD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAGATGATCCGCTCCTT,  downstream forward: _UP4_TAATGACAAAAACTATCATT
References
Reviews
Loading
Original Publications
Loading

FAAC0F819AEC8E055CCEE86D93C8DE5584DC4B23

Page visits: 4159

Time of last update: 2025-04-03 12:59:01

Author of last update: Jstuelk