ahrC

ahrC
168

transcriptional regulator of the ArgR family, AhrC represses the genes for arginine biosynthesis and activates the genes for arginine catabolism

Locus
BSU_24250
Molecular weight
16.69 kDa
Isoelectric point
5.52
Protein length
149 aaSequenceBlast
Gene length
450 bpSequenceBlast
Function
transcriptional regulator of arginine metabolic genes
Product
transcriptional regulator (ArgR family)
Essential
no
Synonyms
ahrC, argR
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1438 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,522,324  2,522,773
Phenotypes of a mutant
increased intracellular concentration of ornithine, citrulline, and arginine PubMed
ahrC inactivation allows growth of a ktrA ktrB kimA mutant at low potassium concentration PubMed
ahrC inactivation allows growth of a proA mutant under conditions of osmotic stress (0.8 M NaCl) PubMed
The protein
Catalyzed reaction/ biological activity
transcriptional activator/ repressor of genes involved in arginine metabolism
Protein family
ArgR family (single member, according to UniProt)
Cofactors
L-arginine is the co-factor required for transcription repression/ activation PubMed
Structure
2P5L (PDB) (complex with an 18bp DNA operator) PubMed
2P5K (PDB) (N-terminus) PubMed
2P5M (PDB) (C-Terminus) PubMed
1F9N (PDB) PubMed
P17893 (AlphaFold)
Effectors of protein activity
L-arginine is the co-factor required for transcription repression/ activation PubMed
Localization
Cytoplasm
Additional information
Binding site consensus: CATGAATAAAAATKCAAK, where K=G or T, PubMed PubMed
Expression and Regulation
Operons
Genes
ahrC-recN
Description
PubMed
Regulation
by sRNA sr1
Additional information
expression is fourfold increased upon depletion of ''nusA'' Reference
Open in new tab

ahrC→recN

2025-03-17 02:47:15

ghost

129

cd9e4583995139b45dec8ee7a4f468ed7762fec6

18832E313ED1EB02E9A9AEB9ED804FDAE6EBBAAD

Genes
dxs-tlyA-ahrC-recN
Description
PubMed
Regulation
Rel dependent downregulation (Class I) during stringent response PubMed
Regulatory mechanism
stringent response: negative regulation, PubMed, in stringent response
Open in new tab

dxs->recN

2025-03-20 04:15:48

Bzhu

145

b0947a7d3105a0852956b3f944c4db191022c978

B8B8483360C3BE8FDAC3C4C5B3BC2ECD2877E42D

Other regulations
Sr1: translation inhibition
Additional information
the sr1 RNA controls ahrC expression via binding to the ahrC mRNA, binding is enhanced by csrA PubMed
Biological materials
Mutant
GP729 (Δ''ahrC::aphA3''), available in Jörg Stülke's lab
GP3122 (Δ''ahrC::phleo''), available in Jörg Stülke's lab
GP2185 (Δ''ahrC::ermC''), available in Jörg Stülke's lab PubMed
BKE24250 (ahrC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTAAGCACCTCTATTTC,  downstream forward: _UP4_TAACAGAAAATCTAACAAAG
BKK24250 (ahrC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTAAGCACCTCTATTTC,  downstream forward: _UP4_TAACAGAAAATCTAACAAAG
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
Labs working on this gene/protein
Simon Phillips, Leeds University, UK Homepage
Michel Debarbouille, Pasteur Institute, Paris, France Homepage
References
Reviews
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Original Publications
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62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4

Page visits: 8788

Time of last update: 2025-04-02 19:04:36

Author of last update: Robert.warneke