FadH

• Description: 2,4-dienoyl-CoA reductase
  
  

Gene name	fadH
Synonyms	ykuF
Essential	no
Product	2,4-dienoyl-CoA reductase
Function	fatty acid degradation
Gene expression levels in SubtiExpress: fadH
Metabolic function and regulation of this protein in SubtiPathways: fadH
MW, pI	26 kDa, 6.495
Gene length, protein length	762 bp, 254 aa
Immediate neighbours	ykuE, fadG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of lipids

This gene is a member of the following regulons

FadR regulon

The gene

Basic information

• Locus tag: BSU14060

Database entries

• BsubCyc: BSU14060

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Trans-2,3-didehydroacyl-CoA + NADP⁺ = trans,trans-2,3,4,5-tetradehydroacyl-CoA + NADPH (according to Swiss-Prot)

• Protein family: 2,4-dienoyl-CoA reductase subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU14060

• Structure:

• UniProt: O34717

• KEGG entry: [3]

• E.C. number: 1.3.1.34

Expression and regulation

• Operon: fadH-fadG PubMed

• Expression browser: fadH PubMed

• Sigma factor:

• Regulation:
  • induced in the presence of long-chain fatty acids (FadR) PubMed

• Regulatory mechanism:
  • FadR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Yasutaro Fujita, Hiroshi Matsuoka, Kazutake Hirooka
Regulation of fatty acid metabolism in bacteria.
Mol Microbiol: 2007, 66(4);829-39
[PubMed:17919287] [WorldCat.org] [DOI] (P p)

Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation.
J Biol Chem: 2007, 282(8);5180-94
[PubMed:17189250] [WorldCat.org] [DOI] (P p)