TagF

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  • Description: CDP-glycerol:polyglycerol phosphate glycero-phosphotransferase

Gene name tagF
Synonyms rodC
Essential yes PubMed
Product CDP-glycerol:polyglycerol phosphate glycero-phosphotransferase
Function biosynthesis of teichoic acid
MW, pI 87 kDa, 9.4
Gene length, protein length 2238 bp, 746 aa
Immediate neighbours tagG, tagE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
TagF context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU35720

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: CDP-glycerol + (glycerophosphate)(n) = CMP + (glycerophosphate)(n+1) (according to Swiss-Prot)
  • Protein family: CDP-glycerol glycerophosphotransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot), membrane associated PubMed

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • repressed when phosphate is available (PhoP) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Edward W C Sewell, Mark P Pereira, Eric D Brown
The wall teichoic acid polymerase TagF is non-processive in vitro and amenable to study using steady state kinetic analysis.
J Biol Chem: 2009, 284(32);21132-8
[PubMed:19520862] [WorldCat.org] [DOI] (P p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

W Liu, S Eder, F M Hulett
Analysis of Bacillus subtilis tagAB and tagDEF expression during phosphate starvation identifies a repressor role for PhoP-P.
J Bacteriol: 1998, 180(3);753-8
[PubMed:9457886] [WorldCat.org] [DOI] (P p)

  1. Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing Bacillus subtilis by divergent approaches. Proteomics 8: 4123-4136 PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed