TagE

• Description: UDP-glucose:polyglycerol phosphate glucosyltransferase
  
  

Gene name	tagE
Synonyms	rodD, gtaA, gtaD
Essential	no
Product	UDP-glucose:polyglycerol phosphate glucosyltransferase
Function	biosynthesis of teichoic acid
MW, pI	78 kDa, 6.026
Gene length, protein length	2019 bp, 673 aa
Immediate neighbours	tagF, tagD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU35730

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: UDP-glucose + poly(glycerol phosphate) = UDP + O-(alpha-D-glucosyl)poly(glycerol phosphate) (according to Swiss-Prot)

• Protein family: glycosyltransferase 1 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-2 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: P13484

• KEGG entry: [3]

• E.C. number: 2.4.1.52

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • repressed when phosphate is available (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

W Liu, S Eder, F M Hulett
Analysis of Bacillus subtilis tagAB and tagDEF expression during phosphate starvation identifies a repressor role for PhoP-P.
J Bacteriol: 1998, 180(3);753-8
[PubMed:9457886] [WorldCat.org] [DOI] (P p)

1. Macek et al. (2007) The serine/ threonine/ tyrosine phosphoproteome of the model bacterium Bacillus subtilis. Mol. Cell. Proteomics 6: 697-707 PubMed
2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed