Sandbox

• Description: D-sorbitol dehydrogenase
  
  

Gene name	gutB
Synonyms
Essential	no
Product	D-sorbitol dehydrogenase
Function	glucitol utilization
MW, pI	38 kDa, 5.213
Gene length, protein length	1059 bp, 353 aa
Immediate neighbours	gutR, gutP
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU06150

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-iditol + NAD⁺ = L-sorbose + NADH (according to Swiss-Prot)

• Protein family: zinc-containing alcohol dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry: Q06004

• KEGG entry: [3]

• E.C. number: 1.1.1.14

Expression and regulation

• Operon: gutP-gutB

• Sigma factor: SigA

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Ng K, Ye R, Wu XC, Wong SL. (1992) Sorbitol dehydrogenase from Bacillus subtilis. Purification, characterization, and gene cloning. J Biol Chem. Dec 15;267(35): 24989-94. PubMed