PolY1

• Description: translesion synthesis (TLS-) DNA polymerase Y1
  
  

Gene name	polY1
Synonyms	yqjH
Essential	no
Product	translesion synthesis (TLS-) DNA polymerase Y1
Function	generation of mutations in stationary phase
MW, pI	46 kDa, 9.953
Gene length, protein length	1242 bp, 414 aa
Immediate neighbours	gndA, yqzJ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU23870

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)

• Protein family: DNA polymerase type-Y family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry: P54545

• KEGG entry: [3]

• E.C. number: 2.7.7.7

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP1111 (spc), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed