RocG

Gene rocG

Codes for RocG, glutamate dehydrogenase

Basic information

Synonyms

Coordinates

DNA sequence

Protein sequence

Essential N

MW

pI

Basic information on the protein

Catalyzed reaction/ biological activity

Protein family

Structure

Swiss prot entry

KEGG entry

Extended information on the protein

Kinetic information

Domains

Modification

Effectors of protein activity

IAX RocG-GltC

Localization

Expression

Operon rocG

Sigma factor SigL

Regulation induced by arginine (RocR-dependent) Repressed by glucose (CcpA-dependent)

Regulatory mechanism RocR: transcription activation, involves long-range DNA looping CcpA: transcription repression

Biological Materials

Mutant GP747, spc (Stülke lab) GP726, aphA3 (Stülke lab)

Expression vector pGP259 (Expression in B. subtilis, based on pBQ200) pGP902 (Strep-tag, based on pGP172) pGP906 (Strep-tag, Expression in B. subtilis, based on pBQ200)

lacZ fusion

GFP fusion

Antibody Stülke lab

Phenotypes of a Mutant

homologous gene(s) in B. subtilis gudB (cryptic glutamate dehydrogenase)

additional remarks