PHT10

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The pHT10 vector contains the coding sequence for a c-Myc tag and allows high-level intracellular production of recombinant c-Myc tagged protein with B. subtilis. The tag can be used for detection or purification of the produced protein of interest. The expression is based on the strong σA-dependent promoter preceding the groE operon of B. subtilis which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. pHT10 is an E. coli / B. subtilis shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis.

Features

• High level production of recombinant proteins
• Efficiently controllable gene expression (IPTG inducible)
• C-terminal c-Myc tag
• E. coli / B. subtilis shuttle vector

pHT10 is a variant of pHT01, both vectors are available from MoBiTec GmbH (Göttingen)