Program
Contents
Sunday June 11, 2017
- 14:00 - 15:00 Registration
- 14:45 - 15:00 – Welcome remarks, Jörg Stülke
Session 1 Sporulation
CHAIR: Sigal Ben-Yehuda
- 15:00 - 15:25 Richard Losick
- Stochasticity and cell-fate determination during sporulation in B. subtilis
- 15:30 - 15:40 Alper Mutlu
- Phenotypic memory links entry and exit from dormancy by a spore quantity-quality tradeoff in Bacillus subtilis
- 15:45 - 15:55 Patrick Eichenberger
- Inferring the Bacillus subtilis global gene regulation network
- 16:00 - 16:10 Tony Wilkinson
- Structures of SpoIIE reveal a regulatory switch shared across the PPC2 phosphatase family and extending to the proteasomal proteases
- 16:15 - 16:25 David Roberts
- Polar segregation of the chromosome origins during sporulation in Bacillus subtilis
- 16:30 - 16:40 Anna Grela
- Functional analysis of GerAA subunit of Bacillus subtilis GerA germination receptor
- 16:45 - 16:55 Eammon Riley
- Defining the metabolic landscape of the Bacillus subtilis sporangium
17:00 Welcome reception
Monday June 12, 2017
Session 2 Cell biology 1
CHAIR: Dirk-Jan Scheffers
- 08:30 - 08:55 Jan Willem Veening
- Identification of a unique cell cycle regulator in Streptococcus pneumoniae by en masse GFP localization and CRISPRi phenotyping
- 09:00 - 09:10 Kumaran Ramamurthi
- An essential Staphylococcus aureus cell division protein regulates both FtsZ assembly and disassembly
- 09:15 - 09:25 Elhanan Tzipilevich
- Acquisition of phage sensitivity by bacteria through exchange of phage receptors
- 09:30 - 09:40 Lauren Cowley
- Evidence for different rates in the mechanisms of recombination in pneumoccal biofilms
- 09:45 - 09:55 Nada Labajova
- Clostridial Min system – possible mechanism of functioning during cell division and sporulation
- 10:00 - 10:10 Kenneth Seistrup
- Lysis induced by membrane depolarisation is caused by MreB-linked mis-regulation of key autolysins in Bacillus subtilis
- 10:15 - 10:45 Coffee break
Session 3 Biotechnology
CHAIR: Oscar Kuipers
- 10:45 - 11:10 Daniel Lopez
- Lipid rafts: From structural and functional characterization to biotechnological application
- 11:15 - 11:25 Marcus Price
- CRISPR-Cas9 mediated engineering of industrially relevant Bacillus subtilis strains
- 11:30 - 11:40 Rocio Aguilar Suarez
- Less is more: towards development of a genome-reduced Bacillus as cell factory for vaccine production
- 11:45 - 11:55 Marta Irla
- Methanol-based production γ-aminobutyric acid in B. methanolicus MGA3
- 12:00 - 12:10 Rita Cruz
- A comparative study of native and heterologous enzyme production in Bacillus subtilis
- 12:30 - 14:00 Lunch
Session 4 New technologies, Systems & Synthetic Biology
CHAIR: Fabian Commichau
- 14:00 - 14:25 Carol Gross
- High throughput phenotyping in Bacillus subtilis
- 14:30 - 14:40 Christopher Zschiedrich
- Large scale identification of evolutionary mechanism that facilitate protein-protein interactions
- 14:45 - 14:55 Leendert Hamoen
- Construction of a minimal divisome reveals robustness of cell division
- 15:00 - 15:10 Etienne Dervyn
- Tailoring Bacillus subtilis-derived chassis for biotechnology applications
- 15:15 - 15:25 Jan Martinussen
- Abrupt glucose depletion in Lactococcus lactis is resulting in an immediate translational stop due to an extreme depletion of the GTP pool
- 15:30 - 15:40 Eugen Peifer
- Adaptive laboratory evolution of Corynebacterium glutamicum towards higher growth rates on glucose minimal medium
- 15:45 - 16:15 Coffee break
Session 5 Cell Biology 2
CHAIR: Stephane Aymerich
- 16:15 - 16:25 Sven Halbedel
- 16:30 - 16:40 Rick Lewis
- Exploring the relationship between penicillin binding proteins and the cell cycle regulator, GpsB
- 16:45 - 16:55 Peter Graumann
- B. subtilis chromosomes are segregated in in a directed diffusion-like manner, likely based on entropic forces, and are condensed by several SMC condensation centres per cell half, as revealed through single molecule tracking
- 17:00 - 17:10 Koichi Yano
- 17:15 - 17:25 Marc Bramkamp
- A dynamin-like protein involved in bacterial cell membrane surveillance under environmental stress
Poster Session 1
- 17:30 - 19:30
Tuesday June 13, 2017
Session Signal transduction I 08:30 - 08:40 Jörgen Johansson Structural and genetic basis for activation and repression of the virulence activator PrfA in Listeria monocytogenes 08:45 - 08:55 Nathalie Declerck Monitoring transcriptional adaptation in live Bacillus subtilis cells using two-photon fluorescence fluctuation microscopy 09:00 - 09:10 Jan Gundlach Cyclic di-AMP controls potassium homeostasis in Bacillus subtilis 09:15 - 09:25 Sylvie Nessler Structural Insights into Streptococcal Competence Regulation by the Cell-to-Cell Communication System ComRS 09:30 - 09:40 Johann Mignolet Ubiquitous rewiring of transcriptional control in streptococci: lesson from competence and predation coupling in Streptococcus salivarius 09:45 - 09:55 Craig Ellermeier RsiV is a bacterial receptor for lysozyme 10:00 - 10:10 Christopher Rao DNA sensing in Bacillus subtilis 10:15 - 10:45 Coffee break Session RNA biology 10:45 - 11:10 Pascale Romby Several regulatory RNAs in Staphylococcus aureus link stress responses, metabolism and virulence factor synthesis
11:15 - 11:25 Ruben Atilho A widespread riboswitch class regulates guanidine metabolism in bacteria 11:30 - 11:40 Emma Denham Towards the in vivo RNA interactome of the Gram positive model organism Bacillus subtilis 12:00 - 12:10 Libor Krasny The Torpedo Effect in Bacillus subtilis: RNase J1 Resolves Stalled Transcription Complexes 12:15 - 12:25 Ciaran Condon Rae1/YacP, a new endoribonuclease involved in ribosome-dependent mRNA decay in B. subtilis 12:30 - 14:00 Lunch Session Metabolism 14:00 - 14:10 Miriam Dormeyer A devil in disguise: a metabolic enzyme converts a transcriptional activator into a repressor 14:15 - 14:25 Lianet Noda-Garcia Mapping of chance and necessity in protein sequence evolution in complex bacterial environments 14:30 - 14:40 Georg Fritz Making and breaking the wall – a systems approach to cell wall homeostasis in Bacillus subtilis 14:45 - 14:55 Christoph Mayer Peptidoglycan recycling in Gram-positive bacteria is crucial for survival 15:00 - 15:10 Amy Bottomley We Are What We Eat: Identifying a Regulatory Crosstalk between Central Carbon Metabolism and Cell Division in Bacteria 15:15 - 15:25 Ines Grilo Glucosaminidase-DNA interaction affects the peptidoglycan hydrolytic activity of glucosaminidase 15:30 - 15:40 Daisuke Seo Purification and characterization of ferredoxin-NADPH oxidoreductase paralogue YcgT in gram-positive bacterium Bacillus subtilis 15:45 - 16:15 Coffee break Session Lifestyles I -Stress 16:15 - 16:40 Jade Wang Regulation of Stress Response and Homeostasis by (p)ppGpp in Bacillus subtilis
16:45 - 16:55 Gerd Bange Structural and functional insights into the (p)ppGpp response of B. subtilis 17:00 - 17:10 Jose A. Lemos The association of metal homeostasis and (p)ppGpp regulation in the pathophysiology of Enterococcus faecalis 17:15 - 17:25 Alexander Reder New insights into the general stress response of Bacillus subtilis - the SigB modulon Poster session 17:30 - 19:30
Wednesday June 14, 2017
Session Lifestyles II - Biofilm 08:30 - 08:40 Polonca Stefanic Kin discrimination foretells B. subtilis lifestyles 08:45 - 08:55 Nozomu Obana Heterogeneity and biofilm morphology in Clostridium perfringens 09:00 - 09:10 Sofia Arnaouteli Bifunctionality of a biofilm matrix protein controlled by redox state 09:15 - 09:25 Anna Dragos Collapse of genetic division of labor and evolution of autonomy in pellicle biofilms 09:30 - 09:40 Tamara Hoffmann Small but essential: the activator protein RemA links biofilm formation and osmostress responses in Bacillus subtilis 09:45 - 09:55 Harald Putzer Swarmer cells lead, multiply and generate a trail of quiescent descendants 10:00 - 10:10 Pascale Beauregard Biofilm and bacillibactin are essential to iron homeostasis in Bacillus subtilis 10:15 - 10:45 Coffee break Session DNA replication and recombination/ Interactions with other organisms 10:45 - 10:55 Mary Anderson Suppression of DNA replication overinitiation through lowered levels of the replicative helicase, DnaC 11:00 - 11:10 Juan Alonso Bacillus subtilis MutS Modulates non-polar recombination between Divergent DNA Sequences 11:15 - 11:25 Paul Straight Antibiotic stimulation of a Bacillus subtilis motile repsonse 11:30 - 11:40 Audrey Labarde Remodelling of the Bacillus subtilis cytoplasm spatial organization for efficient bacteriophage SPP1 multiplication 12:00 - 12:10 Elisabeth Grohmann Molecular insights in conjugative resistance transfer among Gram-positive pathogens 12:15 - 12:25 Javier Pizarro-Cerda A bacteriocin from epidemic Listeria strains alters the host intestinal microbiota to favor infection 12:30 - 14:00 Lunch Session The cereus group 14:00 - 14:25 Didier Lereclus High throughput phenotyping in Bacillus subtilis
14:30 - 14:40 Naomi Bier The Influence of the PEP-PTS on Virulence Gene Expression in Bacillus anthracis 14:45 - 14:55 Sandrine Poncet Hanks-kinases dependent phosphorylation of Bacillus cereus global gene regulator CodY deeply impacts physiology and virulence 15:00 - 15:10 Monika Ehling-Schulz Food Bacteria Interplay: Concerted action of extrinsic and extrinsic factors gearing toxin synthesis in emetic Bacillus cereus 15:15 - 15:25 Joaquin Caro-Astorga Analysis of the molecular machinery implicated in multicellularity in Bacillus cereus 15:30 - 15:40 David Sychantha Structural and mechanistic basis for the O-acetylation of secondary cell wall polysaccharide required for the proper assembly of cell walls in the Bacillus cereus group of human pathogens 15:45 - 16:15 Coffee break Session Pathogenicity/ fighting infection 16:15 - 16:25 Tarek Msadek Bacteriophage mobility and pathogenesis in Staphylococcus aureus 16:30 - 16:40 Van Loi Vu Real-time imaging of the bacillithiol redox potential in the human pathogen Staphylococcus aureus using a genetically encoded bacilliredoxin-fused redox biosensor 16:45 - 16:55 Laty Cahoon A structural comparison of Listeria monocytogenes secretion chaperones PrsA1 and PrsA2 reveals molecular features required for virulence 17:00 - 17:10 Leanne Hays Biofilm formation by the staphylococcal protein SdrC: molecular mechanism and the identification of novel inhibitors 17:15 - 17:25 Emanuel Hanski Integration of host and bacterial signals controls group A streptococcal bacteriocins production to establish dominance in the fascial niche Conference dinner 19:00 - 23:00
Thursday June 15, 2017
Session Lifestyles III 08:30 - 08:40 Sabine Brantl Type I toxin-antitoxin systems from Bacillus subtilis 08:45 - 08:55 Michael Prunty Distinct PhoPR-mediated responses to phosphate limitation in Bacillus subtilis subspecies subtilis and spizizenii stem from differences in their anionic polymer composition and metabolism 09:00 - 09:10 Kursad Turgay Curbing protein synthesis is important for the concerted heat shock response of Bacillus subtilis 09:15 - 09:25 Thorsten Mascher Three's Company: Cannibalism Toxins and the Corresponding Envelope Stress Responses in Bacillus subtilis 09:30 - 09:40 Daniel Rojas Induction of the Spx regulon under cell wall stress requires both an ECF sigma factor and an anti-adaptor protein 09:45 - 09:55 Brandon Anjuwon-Foster The DNA recombinase RecV mediates phase variation of Clostridium difficile flagellum and toxin production 10:00 - 10:10 Ard Jan Grimbergen Bet-hedging strategies in Bacillus subtilis 10:15 - 10:45 Coffee break Session Organizers' favorites 10:45 - 11:10 Emanuelle Charpentier CRISPR-Cas9: a bacterial immune system repurposed as a transformative genome engineering technology
11:15 - 11:25 Ken-Ichi Yoshida Geobacillus kaustophilus Crh is independent of glucose catabolite repression but represses inositol catabolic genes 11:30 - 11:40 Patricia Dos Santos Mutual specificity of cysteine desulfurases and sulfur acceptors in Bacillus subtilis 12:00 - 12:10 Richard Daniel D-ala metabolism in Bacillus subtilis 12:15 - 12:25 Anne-Brit Kolsto The putative drug efflux systems of the Bacillus cereus group 12:30 - 14:00 Lunch
Monday June 22, 2015
- 8:15 - 8:30 – Welcome remarks, Jim Hoch, Marta Perego
- 8:30 - 9:15 - Keynote lecture: Andrew Camilli, Boston, USA
- The Streptococcus pneumoniae cell wall controls pneumolysin release and contributes to virulence.
Session I Signal transduction I
CHAIR: Marta Perego
- 9:15 - 9:30 – Kevin Devine, Dublin, Ireland
- The activities of the WalR-WalK and PhoP-PhoR two-component signal transduction systems are determined by signals that emanate from cell wall metabolism in Bacillus subtilis
- 9:30 - 9:45 – Hendrik Szurmant, La Jolla. USA
- The YycF-YycG two-component system as a regulatory feedback loop for the expression of autolysin activity and the target of antimicrobial activities by human chemokines.
- 9:45 - 10:00 – Tarek Msadek, Paris, France
- Peptide antibiotic resistance and intramembrane kinase signaling in Staphylococcus aureus.
- 10:00 – 10:30 – COFFEE BREAK
Session II Signal transduction II
CHAIR: Kevin Devine
- 10:30 - 10:45 – Mary K. Phillips-Jones, Preston, UK
- Characterization of ligand binding by VanS, the membrane sensor kinase of the two-component system regulating A-type vancomycin resistance in the enterococci.
- 10:45 - 11:00 – Emanuel Hanski, Jerusalem, Israel
- Regulation of group A Streptococcus virulence by sensing of asparagines.
- 11:00 - 11:15 – Nicola Horstmann, Houston, USA
- Regulation of dual-site phosphorylation of response regulator CovR and its impact on group A streptococcus global gene expression and virulence.
- 11:15 - 11:30 – Andrew Ulijasz, London, UK
- A novel family of two-component like redox sensors in the Streptococci.
- 11:30 - 11:45 – Marta Perego, La Jolla, USA
- 11:45 - 12:00 – Maria Cecilia Manilla, Rosario, Argentina
- Proline residues of DesK as transmitting elements of cold stimulus.
- 12:00 - 12:15 – Ken-ichi Yoshida, Kobe, Japan
- 12:30 – 14:00 LUNCH
Parallel sessions
Session IIIA Industrial Research (Sala A)
CHAIR: Ghislain Schyns
- 14:00 - 14:45 – Keynote lecture: Sven Panke, Zürich, Switzerland
- tba
- 14:45 - 15:00 – Abderahmane Derouiche, Gothenburg, Sweden
- 15:00 - 15:15 –Jolanda Neef, Groningen, Netherlands
- A novel expression platform for the production of cell wall-bound and secreted staphylococcal antigens.
- 15:15 - 15:30 – Ferro Iolanda, Potsdam, Germany
- tRNA abundance and aminoacylation in Bacillus licheniformis.
- 15:30 - 15:45 –Birgit Voigt, Greifswald, Germany
- The response of Bacillus pumilus to hydrogen peroxide-provoked oxidative stress.
Session IIIB Host-Pathogen Interaction (Sala B)
CHAIR: Claire Poyart
- 14:00 - 14:15 – Nancy Freitag, Chicago, USA
- Establishing a new niche for bacterial replication: the surface invasin InlB confers cardiotropism to subpopulations of Listeria monocytogenes.
- 14:15 - 14:30 – B. S. Hisey, Bozeman, Montana, USA
- Role of SaeR/S in secondary Staphylococcus aureus pneumonia.
- 14:30 - 14:45 – Anthony Richardson, Chapel Hill, NC, USA
- PPAR-γ is central to the effective host response to Staphylococcus aureus skin and soft tissue infections.
- 14:45 - 15:00 – A.P.A. Hendrickx, Utrecht, Netherlands
- Antibiotics-driven dysbiosis mediate alternative segregation of Enterococcus faecium from the intestinal epithelium.
- 15:00 - 15:15 –Mariana Martins, Paris, France
- Streptococcus gallolyticus Pil3 pilus is required for adhesion to colonic mucus and for colonization of mouse distal colon.
- 15:15 - 15:30 – Salai Madhumathi Parkunan, Oklahoma City, USA
- Bacillus intraocular infection: Divergent roles of IL6 and CXCL1.
- 15:30 - 15:45 – Michelle C. Callegan, Oklahoma City, USA
- The role of pili in Bacillus cereus intraocular infection.
- 15:45 - 16:15 - COFFEE BREAK
Session IV: Vaccines
CHAIR: Guido Grandi
- 16:15 - 16:30 – Fabio Bagnoi, Siena, Italy
- Vaccine composition formulated with a novel TLR7-dependent adjuvant induces high and broad protection against Staphylococcus aureus.
- 16:30 - 16:45 – Olaf Schneewind, Chicago, USA
- Protein A suppresses immune responses during Staphylococcus aureus bloodstream infection in guinea pigs.
- 16:45 - 17:00 – Johannes Hübner, Munich, Germany
- Towards a vaccine against enterococci: Reverse vaccinology and beyond.
- 17:00 - 17:15– Edmondo Campisi, Siena, Italy
- Structural and genetic characterization of the Type IX Group B Streptococcus capsular polysaccharide and its relationship with types V and VII
- 17:15 - 17:30 – Joseph J. Zeppa, London, Ontario, Canada
- Vaccine approaches targeting colonization by Streptococcus pyogenes.
17.30 - 19.30 Poster Session I
Tuesday June 23, 2015
Parallel sessions
Session VA Sporulation (Sala A)
CHAIR: Colin Harwood
- 8:30 - 8:45 – Charles Moran, Atlanta, USA
- Dual-specificity anti-sigma factor reinforces control of cell-type specific gene expression in Bacillus subtilis.
- 8:45 - 9:00 – Adriano Henriques, Oeiras, Portugal
- Single cell analysis of the pathogenicity locus unravels the relationship between toxin production and spore formation in Costridium difficile.
- 9:00 - 9:15 – Adrianne Edwards, Atlanta, USA
- A putative transcription factor containing conserved tetratricopeptide repeat domains controls sporulation, motility and toxin production in Clostridium difficile.
- 9:15 - 9:30 – James R. Walker, Austin, USA
- Genomics of Clostridium taeniosporum, an organism which forms spores with a bundle of ribbon-like appendages.
- 9:30 - 9:45 – Leendert Hamoen, Amsterdam, Netherlands
- Deep starvation reveals a surprisingly active Bacillus subtilis growth state.
- 9:45 - 10:00 – Didier Lereclus, Joyu-en-Josas, France
- Division of labour and terminal differentiation in Bacillus thuringiensis.
- 10:00 - 10:15 – Nils Widderich, Marburg, Germany
- Dysfunctional SigH prevents entry of B. subtilis into sporulation at high salinity.
- 10:15 - 10:30 – Tsutomu Sato, Tokyo, Japan
- Developmentally-regulated prophage excisions reconstitute genes required for sporulation in spore-forming bacteria.
Session VB Antimicrobials/ Drug research (Sala B)
CHAIR: Olaf Schneewind
- 8:30 - 8:45 – Eric A. Johnson, Madison, USA
- Characterization of a novel chimeric Botulinum neurotoxin.
- 8:45 - 9:00 – Oscar Kuipers, Groningen, Netherlands
- Cycling with lantibiotics, and potentiating them against Gram-negative pathogens.
- 9:00 - 9:15 – A. L. Flores-Mireles, St. Louis, USA
- A novel therapy that prevents and resolves E. faecalis catheter-associated urinary tract infections.
- 9:15 - 9:30 – Alexandra Gruss, Jouy-en-Josas, France
- Strategies used by Staphylococcus aureus to bypass FASII-targeted antimicrobials.
- 9:30 - 9:45 – Hanne Ingmer, Frederiksberg, Denmark
- The Staphylococcus aureus agr quorum sensing system as a target for anti-virulence therapy.
- 9:45 - 10:00 – Grace Spatafora, Middlebury, USA
- Characterizing the SloR-DNA binding interface in S. mutans: A target for rational drug design?
- 10:00 - 10:15 – C. Gallay, Groningen, Netherlands
- Lack of pAp phosphatase and inhibition of lipid precursors synthesis lead to mislocalization of key cell division proteins in Streptococcus pneumoniae.
- 10:15 - 10:30 – Yoann Le Breton, College Park, MD, USA
- Using Tn-seq analyses of divergent group A streptococcal isolates for whole-genome studies of fitness and pathogenesis.
- 10:30 - 11:00 - COFFEE BREAK
Session VI: Stress
CHAIR: Indranil Biswas
- 11:00 - 11:15 – Zehava Eichenbaum, Atlanta, USA
- Preventing the red menace: the contributions of streptococcal pefRCD operon in heme stress survival.
- 11:15 - 11:30 – Barbara Spellerberg, Ulm, Germany
- A Streptococcal NRAMP homolog is crucial for the survival of Streptococcus agalactiae under low pH conditions.
- 11:30 - 11:45 – Agnes Fouet, Paris, France
- PerR is necessary for full virulence of Streptococcus agalactiae.
- 11:45 - 12:00 – Melissa E. Reardon-Robinson, Houston, TX, USA
- Oxidative folding of virulence determinants in Gram-positive bacteria.
- 12:30 – 14:00 LUNCH
Session VII: Toxins & antibiotics/ Protection & Resistance
CHAIR: Sandrine Auger
- 13:40 - 13:55 – N. Jahn, Jena, DE
- Against the mainstream: The type I toxin BsrG from Bacillus subtilis does not disrupt membrane potential although it is recruited to the cell membrane
- 14:00 - 14:15 –Yazen Yaseen, Lille, FR
- Comparative study of fengycin promoters using green fluorescent protein (GFP) fusion marker
- 14:20 - 14:35 –Luminita Ciolacu, KEMIN, BE
- Lipopeptides of Bacillus subtilis PB6 - A comparison of two phenotypes
- 14:40 - 14:55—Elrike Frenzel, Groningen, NL
- Single cell profiling of cereulide toxin producing Bacillus cereus
- 15:00 - 15:15 – Anne-Brit Kolstø, Oslo, NO
- The secret lives of bacterial efflux transporter proteins
- 15:20 - 15:35 – Susanne Gebhard, Bath, UK
- Need-based activation of antibiotic resistance by a flux-sensing mechanism
- 15:40 - 16:00 – COFFEE BREAK
Session VIII: Biofilm & Secretion
CHAIR: Kevin Devine
- 16:00 - 16:15 – Akos T Kovacs, University of Jena, DE
- Rise to the surface: importance of motility and aerotaxis in pellicle biofilm development
- 16:20 - 16:35 – Cinzia Calvio, University of Pavia, IT
- Four “y” genes in B. subtilis genome encode γ-PGA hydrolases
- 16:40 - 16:55 – Stavroula Balomenou, University of Crete, GR
- Distinct functions of polysaccharide deacetylases in physiology, virulence and biofilm formation of Bacillus anthracis
- 17:00 - 17:15 – Rita Cruz, DSM Delft, NL
- Impact of secretion and wall stress on protein secretion by Bacillus subtilis
- 17:20 - 17:35 – Alexandria Holland, Bath University, UK
- Protein secretion in Bacillus thermoglucosidasius
- 17:40 - 17:55 – Sara Kesel, University of Munich, DE
- Carbohydrate-coating reduces adhesion of biofilm forming Bacillus subtilis to gold surfaces
- 18:00 Drinks
- 19:00 Dinner