TopB
- Description: DNA topoisomerase III
| Gene name | topB |
| Synonyms | |
| Essential | no |
| Product | DNA topoisomerase III |
| Function | replication |
| Gene expression levels in SubtiExpress: topB | |
| MW, pI | 81 kDa, 9.879 |
| Gene length, protein length | 2181 bp, 727 aa |
| Immediate neighbours | lrpC, ydaJ |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
DNA replication, DNA condensation/ segregation
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU04260
Phenotypes of a mutant
Database entries
- BsubCyc: BSU04260
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to Swiss-Prot)
- Protein family: prokaryotic type I/III topoisomerase family (according to Swiss-Prot)
- Paralogous protein(s): TopA
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU04260
- Structure:
- UniProt: P96583
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- BKE04260 (topB::ermC) (available at the BGSC and in Jörg Stülke's lab)
- GP1864 (topB::ermC) (available in Jörg Stülke's lab)
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Helena B Thomaides, Ella J Davison, Lisa Burston, Hazel Johnson, David R Brown, Alison C Hunt, Jeffery Errington, Lloyd Czaplewski
Essential bacterial functions encoded by gene pairs.
J Bacteriol: 2007, 189(2);591-602
[PubMed:17114254]
[WorldCat.org]
[DOI]
(P p)
