McpC

• Description: membrane-bound chemotaxis receptor for proline, methyl-accepting chemotaxis protein
  
  

Gene name	mcpC
Synonyms	prg71
Essential	no
Product	methyl-accepting chemotaxis protein
Function	control of chemotaxis threonine, glycine, serine, lysine, valine and arginine
Gene expression levels in SubtiExpress: mcpC
Interactions involving this protein in SubtInteract: McpC
MW, pI	71 kDa, 5.174
Gene length, protein length	1962 bp, 654 aa
Immediate neighbours	ykwB, ykwC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

• Locus tag: BSU13950

Phenotypes of a mutant

• not essential for pellicle biofilm formation and mutant has similar fitness to the wild-type strain when competed during pellicle formation PubMed

Database entries

• BsubCyc: BSU13950

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • deaminated McpC stimulates phosphorylation of CheA PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • deamination of Gln-609 (by CheD) PubMed, deaminated McpC stimulates phosphorylation of CheA PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • CheD-McpC, deamination of Gln-609 in McpC PubMed
  • McpC-ArtP, for chemotaxis to lysine and arginine PubMed
  • McpC-MetQ, for chemotaxis to methionine PubMed
  • McpC-YckB, for chemotaxis to tryptophan PubMed

• Localization:
  • cell membrane PubMed

Database entries

• BsubCyc: BSU13950

• Structure:

• UniProt: P54576

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mcpC PubMed

• Expression browser: mcpC PubMed

• Sigma factor: SigD PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • in minimal medium, McpC is present with 2,800 +/- 640 molecules per cell PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 236 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 990 PubMed

Biological materials

• Mutant:
  • DS180 (mcpC::mls in NCIB3610) PubMed
  • TB184 amyE::Phy-sfgfp (mcpC::mls in NCIB3610 with constitutive expressed sfgfp) PubMed
  • TB200 amyE::Phy-mKATE2 (mcpC::mls in NCIB3610 with constitutive expressed mKATE2) PubMed

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed