MurG

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  • Description: UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide)pyrophosphoryl-undecaprenol N-acetylglucosamine transferase, conversion of lipid I to lipid II

Gene name murG
Synonyms
Essential yes PubMed
Product UDP-N-acetylglucosamine-

N-acetylmuramyl-(pentapeptide)pyrophosphoryl-
undecaprenol N-acetylglucosamine transferase

Function peptidoglycan precursor biosynthesis
Gene expression levels in SubtiExpress: murG
Metabolic function and regulation of this protein in SubtiPathways:
murG
MW, pI 39 kDa, 9.568
Gene length, protein length 1089 bp, 363 aa
Immediate neighbours spoVE, murB
Sequences Protein DNA DNA_with_flanks
Genetic context
MurG context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MurG expression.png















Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, sporulation proteins, essential genes, membrane proteins

This gene is a member of the following regulons

SigE regulon, SpoIIID regulon

The gene

Basic information

  • Locus tag: BSU15220

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • converts lipid I to lipid II by attaching GlcNAc to the bactoprenol carrier-conjugated UDP-N-acetylmuramic acid (UDP-MurNAc) pentapeptide molecule
    • UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol (according to Swiss-Prot)
  • Protein family: MurG subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 1F0K (from Escherichia coli, 30% identity, 52% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed during vegatative growth, then switched off, and again expressed during sporulation in the mother cell (SigE) PubMed
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 82 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 627 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References