RecU

• Description: Holliday junction resolvase, DNA repair, homologous recombination and chromosome segregation; recognizes, distorts, and cleaves four-stranded recombination intermediates and modulates RecA activities, equivalent to E. coli RecU, required for efficient survival and replication restart after replication-transcription conflicts
  
  

Gene name	recU
Synonyms	recG, prfA, yppB, jopB
Essential	no
Product	Holliday junction resolvase
Function	DNA repair/ recombination and chromosome segregation
Gene expression levels in SubtiExpress: recU
Interactions involving this protein in SubtInteract: RecU
MW, pI	23 kDa, 9.511
Gene length, protein length	618 bp, 206 aa
Immediate neighbours	yppC, ponA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, cell envelope stress proteins (controlled by SigM, V, W, X, Y)

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

• Locus tag: BSU22310

Phenotypes of a mutant

• the inactivations of recU and ruvA-ruvB are synthetically lethal PubMed

Database entries

• BsubCyc: BSU22310

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • binds Holliday junctions with high affinity PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:
  • RecU-mediated Holliday junction resolution is stimulated by RuvB-ATP PubMed

• Interactions:
  • form dimers PubMed
  • RecA-RecU PubMed
  • RecU-RuvB PubMed
  • formation of a ternary complex RecU-RuvB-Holliday junction DNA PubMed

• Localization:

Database entries

• BsubCyc: BSU22310

• Structure: 1ZP7

• UniProt: P39792

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: recU-ponA PubMed

• Expression browser: recU PubMed

• Sigma factor: SigM PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • GP891 (recU::cat), available in Jörg Stülke's lab
  • 1A895 (no resistance), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

RecU in other organisms

Ana R Pereira, Patricia Reed, Helena Veiga, Mariana G Pinho
The Holliday junction resolvase RecU is required for chromosome segregation and DNA damage repair in Staphylococcus aureus.
BMC Microbiol: 2013, 13;18
[PubMed:23356868] [WorldCat.org] [DOI] (I e)