FloA

• Description: flottilin-like protein (in addition to FloT), resistence protein (against sublancin), accessory role in resistance to cefuroxime
  
  

Gene name	floA
Synonyms	yqfA
Essential	no
Product	flottilin-like protein
Function	resistance against sublancin
Gene expression levels in SubtiExpress: floA
Interactions involving this protein in SubtInteract: FloA
MW, pI	35 kDa, 4.917
Gene length, protein length	993 bp, 331 aa
Immediate neighbours	yqfB, yqeZ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

membrane dynamics, cell envelope stress proteins (controlled by SigM, V, W, X, Y), resistance against toxins/ antibiotics, membrane proteins

This gene is a member of the following regulons

SigW regulon

The gene

Basic information

• Locus tag: BSU25380

Phenotypes of a mutant

• • reduced protein secretion PubMed
  • a floT floA double mutant does not induce KinC-dependent biofilm formation upon addition of surfactin PubMed
  • a floT floA double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency PubMed
  • a floT floA double mutant has a sporulation defect, due to the lack of FtsH PubMed
  • a floA mutant produces increased amount of pulcherriminic acid PubMed
  • a floA mutant has reduced sensitivity to vancomycin PubMed

Database entries

• BsubCyc: BSU25380

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • controls protease activity of FtsH PubMed

• Protein family: UPF0365 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • oligomerization depends on the glutamate-alanine repeats PubMed
  • FloA-FtsH PubMed
  • FloT-FloA PubMed
  • FloA-PhoR PubMed

• Localization:
  • membrane PubMed
  • co-localizes with FloT in discrete foci in the membrane PubMed
  • forms focal structure in the cytoplasma membrane PubMed
  • 13 foci FloA of are detected in strain 3610 PubMed

Database entries

• BsubCyc: BSU25380

• Structure:

• UniProt: P54466

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yqeZ-floA-yqfB PubMed

• Expression browser: floA PubMed

• Sigma factor: SigW PubMed

• Regulation:
  • expressed under stress conditions (SigW) PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 3975 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 3635 PubMed

Biological materials

• Mutant:
  • JS119 (3610 markerless), available in Daniel Lopez's lab
  • DL1401 (168 ΔfloA::mls), available in Daniel Lopez's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:
  • GK192 (3610 lacA::PfloT-yfp(mls)), available in Daniel Lopez's lab
  • JS136 (3610 amyE::floT-gfp(spc)), available in Daniel Lopez's lab
  • JS134 (3610 lacA::floA-mEos2 (mls)), available in Daniel Lopez's lab
  • JS167 (3610 lacA::floA-PAmCherry (mls)), available in Daniel Lopez's lab

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH) for FloA PubMed, available in Daniel Lopez's lab

• Antibody:

References

Reviews

Marc Bramkamp, Daniel Lopez
Exploring the existence of lipid rafts in bacteria.
Microbiol Mol Biol Rev: 2015, 79(1);81-100
[PubMed:25652542] [WorldCat.org] [DOI] (I p)