Program

Tuesday 14th April 2015

▪ 8:00 - 9.00 REGISTRATION ▪ 9:00 - 9:10 – Welcome Introduction

Session I Regulation

CHAIR: Ulrike Mäder 9:10 - 9:25 – Ken-ichi Yoshida, Kobe, Japan ▪ Hyperphosphorylation of DegU cancels CcpA-dependent catabolite repression of rocG in Bacillus subtilis 9:30 - 9:45 – Kambiz Morabbi Heravi, Stuttgart, DE ▪ Activation of the glcR-ywpJ operon by phosphosugar stress in Bacillus subtilis 9:50 - 10:05 – Iolanda Ferro, Potsdam,DE ▪ Regulation of tRNA charging in Bacillus licheniformis and tRNA abundance 10:10 - 10:25 – Ole Økstad, Oslo, NO ▪ Cyclic diguanylate (c-di-GMP) regulation in the Bacillus cereus group - mapping and analysis of putative c-di-GMP effectors 10:30 - 10:45 – Paola Randazzo, Jouy-en-Josas, FR ▪ Transcriptome and proteome analysis of the pulcherrimin-overproducing yvmB Bacillus subtilis mutant

10:50 – 11:15 – COFFEE BREAK

Session II Responses to osmotic stress

CHAIR: Ole Økstad

• 11:20 - 11:35 – Sofia Arnaouteli, Heraklion, GR

▪ Two putative polysaccharide deacetylases are required for osmotic stability and cell shape maintenance in Bacillus anthracis

• 11:40 - 11:55 – S. Löbach, Marburg, DE

▪ Osmotic control of the yqiH-yqiI-yqiK operon by the two component DegS-DegU system of Bacillus subtilis

• 12:00 - 12:15 – Ulrike Mäder, Greifswald, DE

▪ Regulation of opuB expression in Bacillus subtilis involves a SigB-dependent antisense RNA

• 12:20 - 12:35 – Katja Nagler, Cologne, DE

▪ Towards the identification of mechanisms of high-salinity effects on Bacillus subtilis spore germination and outgrowth

• 12:40 – 14:00 LUNCH (+ BACIP MEETING)

Session III: Focus topic: Towards a minimal cell

CHAIR: Jörg Stülke 14:00 - 14:35 – Josef Altenbuchner, Stuttgart, DE ▪ Towards a Bacillus subtilis minimal genome 14:40 - 14:55 – Christopher Sauer, Newcastle, UK ▪ Influence of chromosomal location on the expression of genes in Bacillus subtilis 15:00 - 15:15 –Terrens N. V. Saaki, Amsterdam, NL ▪ Constructing the Bacillus minimal divisome: what regulatory genes are really required for cell division? 15:20 - 15:35 – Etienne Dervyn, Jouy-en-Josas, FR ▪ From knowledge on gene essentiality and transcription to cell factories 15:40 - 16:00 - COFFEE BREAK

Session IV: Stress & sporulation

CHAIR: Imrich Barak 16:00 - 16:15 – Declan Gray, Newcastle, UK ▪ Starvation survival of Bacillus subtilis without sporulation 16:20 - 16:35 – Erwin M. Berendsen, Wageningen, NL ▪ A mobile genetic element is responsible for substantially increased heat resistance of Bacillus subtilis spores 16:40 - 16:55 – Kürsad Turgay, Hannover, DE ▪ Thermotolerance development in Bacillus subtilis 17:00 - 17:15– Marina Raguse, Cologne, DE ▪ Plasma sterilization as an innovative tool to inactive Bacillus subtilis endospores 17:20 - 17:35 – Alexander Reder, Greifswald, DE ▪ Issues of competence – the general stress sigma factor SigB silences competence development in Bacillus subtilis 17.40 - 17.55—Jan Maarten van Dijl, Groningen, NL ▪ Ribosome remodeling in ethanol-stressed cells of Bacillus subtilis is mediated by a SigB-dependent antisense RNA 18.00 - 18.15—Sylvie Nessler, University Paris-Sud, FR ▪ Bacillus subtilis serine/threonine protein kinase YabT

Wednesday April 15th 2015

Session V: Tools et al.

CHAIR: Stephane Aymerich 9:00 - 9:15 – Jara Radeck, Munich, DE ▪ The Bacillus BioBrick Box 2.0: Generation and Evaluation of New Essential Genetic Building Blocks for Standardized Work with Bacillus subtilis 9:20 - 9:35 – Wilfried J. J. Meijer, Madrid, ES ▪ Studying Bacillus subtilis plasmid conjugation and its use as a tool for modifying other relevant Gram positive bacteria 9:40 - 9:55 – Trupti Sharma, Bath, UK ▪ Microbiologically induced calcium carbonate precipitation by extremophilic Bacillus species – A potential application as microbial agents for the development of self-healing concrete 10:00 - 10:35 - COFFEE BREAK

Session VI: Cell wall and cell biology

CHAIR: Uwe Völker 10:40 - 10:55 – Mike van Nieuwenhze, Bloomington/ Newcastle, USA/ UK ▪ Novel chemical tools for probing the dynamics of peptidoglycan biosynthesis 11:00 - 11:15 – Henrik Strahl, Newcastle, UK ▪ Polar localization of bacterial chemoreceptors by recruitment to highly curved septal membrane 11:20 - 11:35 – David Noone, Trinity College Dublin, IE ▪ The WalK sensor kinase detects several signals during the growth cycle of Bacillus subtilis 11:40 - 11:55 – Karzan R. Sidiq, Newcastle, UK ▪ A D-alanine transporter in Bacillus subtilis 12:00 - 12:15 – Peter Graumann, Marburg, DE ▪ MreB forms curved sheets of filaments that nucleate on membranes in vitro 12:20 - 12:35 – Jeanine Rismondo, Wernigerode, DE ▪ Discrete and overlapping roles of the five high molecular weight penicillin binding proteins in cell division, growth and virulence of Listeria monocytogenes 12:40 - 13:40 – LUNCH

Session VII: Toxins & antibiotics/ Protection & Resistance

CHAIR: Sandrine Auger 13:40 - 13:55 – N. Jahn, Jena, DE ▪ Against the mainstream: The type I toxin BsrG from Bacillus subtilis does not disrupt membrane potential although it is recruited to the cell membrane 14:00 - 14:15 –Yazen Yaseen, Lille, FR ▪ Comparative study of fengycin promoters using green fluorescent protein (GFP) fusion marker 14:20 - 14:35 –Luminita Ciolacu, KEMIN, BE ▪ Lipopeptides of Bacillus subtilis PB6 - A comparison of two phenotypes 14:40 - 14:55—Elrike Frenzel, Groningen, NL ▪ Single cell profiling of cereulide toxin producing Bacillus cereus 15:00 - 15:15 – Anne-Brit Kolstø, Oslo, NO ▪ The secret lives of bacterial efflux transporter proteins 15:20 - 15:35 – Susanne Gebhard, Bath, UK ▪ Need-based activation of antibiotic resistance by a flux-sensing mechanism 15:40 - 16:00 – COFFEE BREAK

Session VIII: Biofilm & Secretion

CHAIR: Kevin Devine 16:00 - 16:15 – Akos Kovacs, University of Jena, DE ▪ Living on the surface: Biofilm formation and surface motility of Bacillus subtilis 16:20 - 16:35 – Cinzia Calvio, University of Pavia, IT ▪ Four “y” genes in B. subtilis genome encode γ-PGA hydrolases 16:40 - 16:55 – Stavroula Balomenou, University of Crete, GR ▪ Distinct functions of polysaccharide deacetylases in physiology, virulence and biofilm formation of Bacillus anthracis 17:00 - 17:15 – Rita Cruz, DSM Delft, NL ▪ Impact of secretion and wall stress on protein secretion by Bacillus subtilis 17:20 - 17:35 – Alexandria Holland, Bath University, UK ▪ Protein secretion in Bacillus thermoglucosidasius 17:40 - 17:55 – Sara Kesel, University of Munich, DE ▪ Carbohydrate-coating reduces adhesion of biofilm forming Bacillus subtilis to gold surfaces

18:00 Drinks

19:00 Dinner