YqhY
- Description: unknown
Gene name | yqhY |
Synonyms | |
Essential | no PubMed |
Product | unknown |
Function | unknown |
Gene expression levels in SubtiExpress: yqhY | |
Metabolic function and regulation of this protein in SubtiPathways: yqhY | |
MW, pI | 14 kDa, 4.414 |
Gene length, protein length | 405 bp, 135 aa |
Immediate neighbours | nusB, accC |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU24330
Phenotypes of a mutant
Database entries
- BsubCyc: BSU24330
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: asp23 family (according to Swiss-Prot)
- Paralogous protein(s): YloU
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU24330
- Structure:
- UniProt: P54519
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
- number of protein molecules per cell (minimal medium with glucose and ammonium): 127 PubMed
- number of protein molecules per cell (complex medium with amino acids, without glucose): 893 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 4905 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 3644 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 4928 PubMed
Biological materials
- Mutant:
- GP1468 (yqhY::erm), available in Jörg Stülke's lab PubMed
- GP1474 (chromosomal yqhY-Strep fusion, aphA3), purification from B. subtilis, for SPINE, available in Jörg Stülke's lab
- Expression vector:
- pGP1322 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
- pGP1325 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
- pGP1496 (His-tag, purification from E. coli, in pET28a+), available in Jörg Stülke's lab
- pGP1498 (N-terminal His-tag, TEV-site, purification from E. coli, in pWH844), available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- GP1471 (spc, based on pBP43), available in Jörg Stülke's lab
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- FLAG-tag construct:
- GP1481 (spc, based on pGP1331), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References