BshC

• Description: cysteine-adding enzyme required for the synthesis of bacillithiol
  
  

Gene name	bshC
Synonyms	yllA
Essential	no
Product	cysteine-adding enzyme
Function	biosynthesis of bacillithiol
Gene expression levels in SubtiExpress: bshC
MW, pI	62 kDa, 5.247
Gene length, protein length	1617 bp, 539 aa
Immediate neighbours	ylbQ, mraZ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, resistance against oxidative and electrophile stress

This gene is a member of the following regulons

Spx regulon

The gene

Basic information

• Locus tag: BSU15120

Database entries

• BsubCyc: BSU15120

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU15120

• Structure: 4WBD PubMed

• UniProt: P55342

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ylbQ-bshC PubMed

• Expression browser: bshC PubMed

• Sigma factor:
  • ylbQ: SigA PubMed
  • bshC: SigA PubMed

• Regulation:
  • ylbQ: induced by diamide stess (thiol depletion) (Spx) PubMed

• Regulatory mechanism:
  • Spx: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 212 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 717 PubMed

Biological materials

• Mutant: bshC null mutant available in John Helmann lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: