BcaP
- Description: branched-chain amino acid transporter, major transporter for isoleucine and valine, may also be involved in threonine transport
Gene name | bcaP |
Synonyms | yhdG |
Essential | no |
Product | branched-chain amino acid transporter |
Function | biosynthesis/acquisition of branched-chain amino acids |
Gene expression levels in SubtiExpress: bcaP | |
Metabolic function and regulation of this protein in SubtiPathways: BcaP | |
MW, pI | 49 kDa, 9.735 |
Gene length, protein length | 1395 bp, 465 aa |
Immediate neighbours | yhdF, yhdH |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
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Expression at a glance PubMed |
Contents
Categories containing this gene/protein
transporters/ other, biosynthesis/ acquisition of amino acids, utilization of amino acids, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU09460
Phenotypes of a mutant
- no phenotype for the single mutant, the triple bcaP brnQ braB mutant is strongly impaired in the transport of isoleucine and valine at low concentrations PubMed
- resistant to growth inhibition by high concentrations of threonine PubMed
Database entries
- BsubCyc: BSU09460
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- high affinity uptake of isoleucine and valine PubMed
- Paralogous protein(s): YfnA
Extended information on the protein
- Kinetic information:
- for Ile: Km ≈ 4.1 μM; Vmax at 26°C was ~15 nmol/min/ mg protein PubMed
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cell membrane PubMed
Database entries
- BsubCyc: BSU09460
- Structure:
- UniProt: O07576
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Additional information:
Biological materials
- Mutant: BKE09460 (bcaP::erm, available in the BGSC and in Fabian Commichau's lab)
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References