CheC

• Description: control of chemotaxis by interacting with CheD, CheY-P phosphatase, inhibition of CheR-mediated methylation of MCPs
  
  

Gene name	cheC
Synonyms	ylxJ
Essential	no
Product	CheY-P phosphatase
Function	motility and chemotaxis
Gene expression levels in SubtiExpress: cheC
Interactions involving this protein in SubtInteract: CheC
MW, pI	22 kDa, 4.035
Gene length, protein length	627 bp, 209 aa
Immediate neighbours	cheW, cheD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, motility and chemotaxis

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU16450

Database entries

• BsubCyc: BSU16450

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • CheY-P phosphatase
  • controls binding of CheD to chemoreceptors in response to the levels of CheY-P PubMed

• Protein family: cheC family (according to Swiss-Prot)

• Paralogous protein(s):FliY

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: interaction with CheD enhances phosphatase activity towards CheY-P PubMed

• Interactions:
  • McpB-CheC PubMed
  • CheA-CheC PubMed
  • CheC-CheD PubMed, this interaction is enhanced by CheY-P PubMed
  • CheC-CheY-P

• Localization:

Database entries

• BsubCyc: BSU16450

• Structure:

• UniProt: P40403

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
  • ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed

• Expression browser: cheC PubMed

• Sigma factor:
  • flgB: SigA PubMed, SigD PubMed
  • ylxF: SigD PubMed

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed
  • CodY: transcription repression PubMed
  • DegU (non-phosphorylated): transcription activation PubMed
  • DegU-P: transcription repression PubMed
  • DegU-P in complex with SwrA: transcription activation PubMed

• Additional information:
  • in minimal medium, CheC is present with 770 +/- 330 molecules per cell PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 152 PubMed

Biological materials

• Mutant:
  • 1A862 (no resistance), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: