PdxT

• Description: pyridoxal-5'-phosphate synthase (glutaminase domain)
  
  

Gene name	pdxT
Synonyms	yaaE
Essential	no
Product	pyridoxal-5'-phosphate synthase (glutaminase domain)
Function	pyridoxal-5'-phosphate biosynthesis
Gene expression levels in SubtiExpress: pdxT
Interactions involving this protein in SubtInteract: PdxT
Metabolic function and regulation of this protein in SubtiPathways: PdxT
MW, pI	21 kDa, 4.984
Gene length, protein length	588 bp, 196 aa
Immediate neighbours	pdxS, serS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

• Locus tag: BSU00120

Database entries

• BsubCyc: BSU00120

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: glutamine amidotransferase pdxT/SNO family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • PdxS-PdxT PubMed

• Localization:

Database entries

• BsubCyc: BSU00120

• Structure:
  • PdxT 2NV0 PubMed
  • PdxS-PdxT complex 2NV2 PubMed

• UniProt: P37528

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: pdxS-pdxT PubMed

• Expression browser: pdxT PubMed

• Sigma factor:

• Regulation: negatively controlled by Spo0A PubMed
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1683 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 3958 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 749 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 443 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 55 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Miriam Dormeyer, Richard Egelkamp, Martin J Thiele, Elke Hammer, Katrin Gunka, Lorena Stannek, Uwe Völker, Fabian M Commichau
A novel engineering tool in the Bacillus subtilis toolbox: inducer-free activation of gene expression by selection-driven promoter decryptification.
Microbiology (Reading): 2015, 161(Pt 2);354-361
[PubMed:25473090] [WorldCat.org] [DOI] (I p)

Shiori Itagaki, Minami Haga, Yuji Oikawa, Ayaka Sakoda, Yoshie Ohke, Hiroshi Sawada, Tadashi Eguchi, Hideyuki Tamegai
Differences in the roles of a glutamine amidotransferase subunit of pyridoxal 5'-phosphate synthase between Bacillus circulans and Bacillus subtilis.
Biosci Biotechnol Biochem: 2013, 77(7);1481-5
[PubMed:23832367] [WorldCat.org] [DOI] (I p)

Silvia Wallner, Martina Neuwirth, Karlheinz Flicker, Ivo Tews, Peter Macheroux
Dissection of contributions from invariant amino acids to complex formation and catalysis in the heteromeric pyridoxal 5-phosphate synthase complex from Bacillus subtilis.
Biochemistry: 2009, 48(9);1928-35
[PubMed:19152323] [WorldCat.org] [DOI] (I p)

Marco Strohmeier, Thomas Raschle, Jacek Mazurkiewicz, Karsten Rippe, Irmgard Sinning, Teresa B Fitzpatrick, Ivo Tews
Structure of a bacterial pyridoxal 5'-phosphate synthase complex.
Proc Natl Acad Sci U S A: 2006, 103(51);19284-9
[PubMed:17159152] [WorldCat.org] [DOI] (P p)

Thomas Raschle, Nikolaus Amrhein, Teresa B Fitzpatrick
On the two components of pyridoxal 5'-phosphate synthase from Bacillus subtilis.
J Biol Chem: 2005, 280(37);32291-300
[PubMed:16030023] [WorldCat.org] [DOI] (P p)

Jianghai Zhu, John W Burgner, Etti Harms, Boris R Belitsky, Janet L Smith
A new arrangement of (beta/alpha)8 barrels in the synthase subunit of PLP synthase.
J Biol Chem: 2005, 280(30);27914-23
[PubMed:15911615] [WorldCat.org] [DOI] (P p)

Boris R Belitsky
Physical and enzymological interaction of Bacillus subtilis proteins required for de novo pyridoxal 5'-phosphate biosynthesis.
J Bacteriol: 2004, 186(4);1191-6
[PubMed:14762015] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)