DarA

• Description: c-di-AMP-binding PII-like protein
  
  

Gene name	darA
Synonyms	yaaQ
Essential
Product	c-di-AMP-binding PII-like protein
Function	unknown
Gene expression levels in SubtiExpress: darA
MW, pI	11 kDa, 6.088
Gene length, protein length	327 bp, 109 aa
Immediate neighbours	tmk, yaaR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

proteins of unknown function

The gene

Basic information

• Locus tag: BSU00290

Database entries

• BsubCyc: BSU00290

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:
  • binds c-di-AMP PubMed

• Interactions:
  • forms homo-trimers PubMed

• Localization:

Database entries

• BsubCyc: BSU00290

• Structure:
  • 4RLE (the B. subtilis protein in complex with c-di-AMP) PubMed
  • 3M05 (the apoprotein from Pediococcus pentosaceus, 50% identity)

• UniProt: P37538

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: darA-yaaR-holB-yaaT-yabA-yabB-yazA-yabC PubMed

• Expression browser: darA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1030 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 922 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 894 PubMed

Biological materials

• Mutant:
  • GP1712 (darA::cat), available in Jörg Stülke's lab PubMed
  • GP1713 (darA-yaaR::cat), available in Jörg Stülke's lab

• Expression vector:
  • pGP2601: IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab PubMed
  • pGP2624: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
  • pGP2603: expression of yaaQ-Strep by pGP382 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

• Antibody: available in Jörg Stülke's lab