SsrA

• Description: tmRNA
  
  

Gene name	ssrA
Synonyms
Essential	no
Product	tmRNA
Function	trans-translation
Gene length	bp
Immediate neighbours	yvaG, smpB
Gene sequence (+200bp)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

translation, ncRNA

The gene

Basic information

• Locus tag:

• Interactions: SmpB-SsrA

Phenotypes of a mutant

• impaired growth at high and low temperatures PubMed
• inactivation of ssrA restores beta-lactam resistance in a sigM mutant PubMed

Database entries

• BsubCyc: BSU_misc_RNA_55

• DBTBS entry: no entry

• SubtiList entry: [1]

• Structure:

Expression and regulation

• Operon:
  • secG-yvaK-rnr-smpB-ssrA PubMed
  • yvaK-rnr-smpB-ssrA PubMed
  • smpB-ssrA PubMed
  • ssrA PubMed

• Sigma factor:
  • yvaK: SigB PubMed

• Regulation:
  • yvaK: induced by stress (SigB) PubMed

• Additional information:
  • maturation of tmRNA on the 3' and 5' ends depends on RNase Z and rnpB, respectively PubMed

Biological materials

• Mutant: GP323 (cat), available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/RNA

• Chet Price, Davis, USA homepage

References

Reviews

Noël Molière, Kürşad Turgay
General and regulatory proteolysis in Bacillus subtilis.
Subcell Biochem: 2013, 66;73-103
[PubMed:23479438] [WorldCat.org] [DOI] (P p)

Janine Kirstein, Noël Molière, David A Dougan, Kürşad Turgay
Adapting the machine: adaptor proteins for Hsp100/Clp and AAA+ proteases.
Nat Rev Microbiol: 2009, 7(8);589-99
[PubMed:19609260] [WorldCat.org] [DOI] (I p)

Jeffrey H Withey, David I Friedman
A salvage pathway for protein structures: tmRNA and trans-translation.
Annu Rev Microbiol: 2003, 57;101-23
[PubMed:12730326] [WorldCat.org] [DOI] (P p)

Original publications

Laetitia Gilet, Jeanne M DiChiara, Sabine Figaro, David H Bechhofer, Ciarán Condon
Small stable RNA maturation and turnover in Bacillus subtilis.
Mol Microbiol: 2015, 95(2);270-82
[PubMed:25402410] [WorldCat.org] [DOI] (I p)

Yun Luo, John D Helmann
Analysis of the role of Bacillus subtilis σ(M) in β-lactam resistance reveals an essential role for c-di-AMP in peptidoglycan homeostasis.
Mol Microbiol: 2012, 83(3);623-39
[PubMed:22211522] [WorldCat.org] [DOI] (I p)

Hiromi Ujiie, Tomoko Matsutani, Hisashi Tomatsu, Ai Fujihara, Chisato Ushida, Yasuhiko Miwa, Yasutaro Fujita, Hyouta Himeno, Akira Muto
Trans-translation is involved in the CcpA-dependent tagging and degradation of TreP in Bacillus subtilis.
J Biochem: 2009, 145(1);59-66
[PubMed:18977770] [WorldCat.org] [DOI] (I p)

Ji-Hyun Shin, Chester W Price
The SsrA-SmpB ribosome rescue system is important for growth of Bacillus subtilis at low and high temperatures.
J Bacteriol: 2007, 189(10);3729-37
[PubMed:17369301] [WorldCat.org] [DOI] (P p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

Marc A B Kolkman, Eugenio Ferrari
The fate of extracellular proteins tagged by the SsrA system of Bacillus subtilis.
Microbiology (Reading): 2004, 150(Pt 2);427-436
[PubMed:14766921] [WorldCat.org] [DOI] (P p)

T Wiegert, W Schumann
SsrA-mediated tagging in Bacillus subtilis.
J Bacteriol: 2001, 183(13);3885-9
[PubMed:11395451] [WorldCat.org] [DOI] (P p)

A W Karzai, E D Roche, R T Sauer
The SsrA-SmpB system for protein tagging, directed degradation and ribosome rescue.
Nat Struct Biol: 2000, 7(6);449-55
[PubMed:10881189] [WorldCat.org] [DOI] (P p)