YjbH

• Description: adaptor protein for ClpX-ClpP-catalyzed Spx degradation, confers resistance against nitrosating agents
  
  

Gene name	yjbH
Synonyms
Essential	no
Product	adaptor protein
Function	stimulation of Spx degradation
Gene expression levels in SubtiExpress: yjbH
Interactions involving this protein in SubtInteract: YjbH
MW, pI	31 kDa, 5.206
Gene length, protein length	825 bp, 275 aa
Immediate neighbours	yizD, yjbI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

proteolysis, resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)

The gene

Basic information

• Locus tag: BSU11550

Phenotypes of a mutant

• increased thermotolerance due to increased stabiliy of Spx and thus increased expression of trxA PubMed

Database entries

• BsubCyc: BSU11550

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: adaptor protein for ClpX-ClpP-catalyzed Spx degradation PubMed

• Protein family: UPF0413 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:
  • contains Zn atoms (coordinated by the N-terminal His-rich region) PubMed

• Effectors of protein activity:
  • YjbH aggregates under stress conditions, resulting in the inability to bind to Spx, and therefore in stabilization of Spx PubMed
  • Zn atom is released upon treatment with strong oxidants PubMed
  • interaction with YirB inhibits the formation of a complex with Spx PubMed

• Interactions:
  • YjbH-Spx PubMed
  • YirB-YjbH PubMed

• Localization: cytosolic protein

Database entries

• BsubCyc: BSU11550

• Structure:

• UniProt: O31606

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yjbI-yjbH PubMed

• Expression browser: yjbH PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • A ncRNA is predicted between yizD and yjbH PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Peter Zuber, Oregon Health and Science University, USA Homepage

Claes von Wachenfeldt, Lund University, Sweden Homepage