CotB

• Description: spore coat protein (outer)
  
  

Gene name	cotB
Synonyms
Essential	no
Product	spore coat protein (outer)
Function	resistance of the spore
Gene expression levels in SubtiExpress: cotB
Interactions involving this protein in SubtInteract: CotB
MW, pI	42 kDa, 9.831
Gene length, protein length	1140 bp, 380 aa
Immediate neighbours	ywrJ, cotH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

GerE regulon, GerR regulon, SigK regulon, SigE regulon

The gene

Basic information

• Locus tag: BSU36050

Database entries

• BsubCyc: BSU36050

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • CotB-CotG PubMed

• Localization:
  • outer spore coat, at the middle of the spore as a ring- or spiral-like structure, localization depends on CotE PubMed

Database entries

• BsubCyc: BSU36050

• Structure:

• UniProt: P07789

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: cotB PubMed

• Sigma factor: SigE PubMed, SigK PubMed,

• Regulation:
  • expressed late during sporulation in the mother cell (SigE, SigK, GerE, GerR) PubMed

• Regulatory mechanism:
  • GerE: transcription activation PubMed
  • GerR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:
  • 1S102 ( cotB::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Marco Plomp, Alicia Monroe Carroll, Peter Setlow, Alexander J Malkin
Architecture and assembly of the Bacillus subtilis spore coat.
PLoS One: 2014, 9(9);e108560
[PubMed:25259857] [WorldCat.org] [DOI] (I e)

Peter T McKenney, Patrick Eichenberger
Dynamics of spore coat morphogenesis in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);245-60
[PubMed:22171814] [WorldCat.org] [DOI] (I p)

Giuseppina Cangiano, Antonio Mazzone, Loredana Baccigalupi, Rachele Isticato, Patrick Eichenberger, Maurilio De Felice, Ezio Ricca
Direct and indirect control of late sporulation genes by GerR of Bacillus subtilis.
J Bacteriol: 2010, 192(13);3406-13
[PubMed:20435725] [WorldCat.org] [DOI] (I p)

Daisuke Imamura, Ritsuko Kuwana, Hiromu Takamatsu, Kazuhito Watabe
Localization of proteins to different layers and regions of Bacillus subtilis spore coats.
J Bacteriol: 2010, 192(2);518-24
[PubMed:19933362] [WorldCat.org] [DOI] (I p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

Rita Zilhão, Mónica Serrano, Rachele Isticato, Ezio Ricca, Charles P Moran, Adriano O Henriques
Interactions among CotB, CotG, and CotH during assembly of the Bacillus subtilis spore coat.
J Bacteriol: 2004, 186(4);1110-9
[PubMed:14762006] [WorldCat.org] [DOI] (P p)

L Zheng, R Halberg, S Roels, H Ichikawa, L Kroos, R Losick
Sporulation regulatory protein GerE from Bacillus subtilis binds to and can activate or repress transcription from promoters for mother-cell-specific genes.
J Mol Biol: 1992, 226(4);1037-50
[PubMed:1518043] [WorldCat.org] [DOI] (P p)

L B Zheng, R Losick
Cascade regulation of spore coat gene expression in Bacillus subtilis.
J Mol Biol: 1990, 212(4);645-60
[PubMed:1691789] [WorldCat.org] [DOI] (P p)

W Donovan, L B Zheng, K Sandman, R Losick
Genes encoding spore coat polypeptides from Bacillus subtilis.
J Mol Biol: 1987, 196(1);1-10
[PubMed:2821284] [WorldCat.org] [DOI] (P p)