SsbB

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  • Description: single-strand DNA-binding proteinrequired to internalize and to recombine ssDNA with homologous resident duplex

Gene name ssbB
Synonyms ywpH
Essential no
Product single-strand DNA-binding protein
Function required for efficient genetic transformation
Gene expression levels in SubtiExpress: ssbB
Interactions involving this protein in SubtInteract: SsbB
MW, pI 12 kDa, 6.876
Gene length, protein length 339 bp, 113 aa
Immediate neighbours glcR, ywpG
Sequences Protein DNA DNA_with_flanks
Genetic context
YwpH context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SsbB expression.png















Categories containing this gene/protein

DNA replication, genetic competence, DNA repair/ recombination, phosphoproteins

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

  • Locus tag: BSU36310

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • binds and protects internalized foreign ssDNA PubMed
  • Protein family:
  • Paralogous protein(s): SsbA

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity: phosphorylation strongly increases DNA-binding activity PubMed
  • Localization:
    • localizes to cell poles in competent cells PubMed
    • localizes to the DNA entry pole during transformation PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed at the onset of stationary phase PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 144 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 617 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 473 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1758 PubMed

Biological materials

  • Mutant:
  • Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

Original publications