PbpB

• Description: class B penicillin-binding protein 2B, Pbp2B
  
  

Gene name	pbpB
Synonyms
Essential	yes PubMed
Product	penicillin-binding protein 2B
Function	septation, cell division
Gene expression levels in SubtiExpress: pbpB
Interactions involving this protein in SubtInteract: PbpB
MW, pI	79 kDa, 9.234
Gene length, protein length	2148 bp, 716 aa
Immediate neighbours	ftsL, spoVD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, cell division, essential genes, membrane proteins

The gene

Basic information

• Locus tag: BSU15160

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU15160

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: transpeptidase family (according to Swiss-Prot)

• Paralogous protein(s): SpoVD

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • folding requires PrsA PubMed
  • PbpB-DivIB-(FtsL-DivIC)2 PubMed, as part of the divisome
  • WalK-PbpB PubMed

• Localization:
  • cell membrane PubMed
  • division sites PubMed
  • extracellular (signal peptide) PubMed
  • localizes to the sporulation septum during sporulation PubMed

Database entries

• BsubCyc: BSU15160

• Structure: 1RP5 (PBP 2x from Streptococcus pneumoniae, 32% identity) PubMed

• UniProt: Q07868

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mraZ-mraW-ftsL-pbpB PubMed

• Expression browser: pbpB PubMed

• Sigma factor:

• Regulation:
  • constitutively expressed PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab

• FLAG-tag construct:
  • GP2003 pbpB-3xFLAG spec (based on pGP1331), available in Jörg Stülke's lab

• Antibody:

Labs working on this gene/protein

Jeff Errington, Newcastle University, UK homepage