MraY

• Description: phospho-N-acetylmuramoyl-pentapeptide-transferase (meso-2,6-diaminopimelate), catalyzes the first commited membrane-bound step of bacterial peptidoglycan synthesis leading to the formation of lipid I
  
  

Gene name	mraY
Synonyms
Essential	yes PubMed
Product	phospho-N-acetylmuramoyl-pentapeptide-transferase (meso-2,6-diaminopimelate)
Function	peptidoglycan precursor biosynthesis
Gene expression levels in SubtiExpress: mraY
Metabolic function and regulation of this protein in SubtiPathways: mraY
MW, pI	35 kDa, 8.966
Gene length, protein length	972 bp, 324 aa
Immediate neighbours	murE, murD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, essential genes, membrane proteins

The gene

Basic information

• Locus tag: BSU15190

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU15190

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol (according to Swiss-Prot)

• Protein family: MraY subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU15190

• Structure:

• UniProt: Q03521

• KEGG entry: [3]

• E.C. number: 2.7.8.13

Expression and regulation

• Operon: murE-mraY-murD-spoVE-murG-murB-divIB-ylxW-ylxX-sbp PubMed

• Expression browser: mraY PubMed

• Sigma factor:

• Regulation:
  • expressed during vegatative growth PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• FLAG-tag construct:
  • GP2001 mraY-3xFLAG ermC (based on pGP1087), available in Jörg Stülke's lab

• Antibody: