YvcK
- Description: essential for growth under gluconeogenetic conditions, required for correct localization of PBP1
Gene name | yvcK |
Synonyms | |
Essential | no |
Product | unknown |
Function | localization of PBP1 |
Gene expression levels in SubtiExpress: yvcK | |
Interactions involving this protein in SubtInteract: YvcK | |
MW, pI | 34 kDa, 5.408 |
Gene length, protein length | 951 bp, 317 aa |
Immediate neighbours | yvcL, yvcJ |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
carbon core metabolism, cell shape, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU34760
Phenotypes of a mutant
- unable to grow with gluconeogenic substrates as single carbon source PubMed
- filamentous or L-shape-like aberrant morphologies (suppressed by Mg++) PubMed, this is supressed by overexpression of MreB or by deletion of ponA (PBP1 ) PubMed
Database entries
- BsubCyc: BSU34760
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: UPF0052 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: localized as a helical-like pattern PubMed
Database entries
- BsubCyc: BSU34760
- Structure:
- UniProt: O06974
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation: very weak stimuation of expression by citrate and succinate PubMed
- Regulatory mechanism:
- Additional information:
- translation is likely to require Efp due to the presence of several consecutive proline residues PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium): 96 PubMed
- number of protein molecules per cell (complex medium with amino acids, without glucose): 246 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 555 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 282 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 459 PubMed
Biological materials
- Mutant:
- Expression vector: pGP736 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
- Antibody:
Labs working on this gene/protein
Boris Görke, University of Göttingen, Germany Homepage
Anne Galinier, University of Marseille, France
Your additional remarks
References
Reviews
Original publications
Mushtaq Mir, Sladjana Prisic, Choong-Min Kang, Shichun Lun, Haidan Guo, Jeffrey P Murry, Eric J Rubin, Robert N Husson
Mycobacterial gene cuvA is required for optimal nutrient utilization and virulence.
Infect Immun: 2014, 82(10);4104-17
[PubMed:25047842]
[WorldCat.org]
[DOI]
(I p)
Elodie Foulquier, Frédérique Pompeo, Céline Freton, Baptiste Cordier, Christophe Grangeasse, Anne Galinier
PrkC-mediated phosphorylation of overexpressed YvcK protein regulates PBP1 protein localization in Bacillus subtilis mreB mutant cells.
J Biol Chem: 2014, 289(34);23662-9
[PubMed:25012659]
[WorldCat.org]
[DOI]
(I p)
Elodie Foulquier, Frédérique Pompeo, Alain Bernadac, Leon Espinosa, Anne Galinier
The YvcK protein is required for morphogenesis via localization of PBP1 under gluconeogenic growth conditions in Bacillus subtilis.
Mol Microbiol: 2011, 80(2);309-18
[PubMed:21320184]
[WorldCat.org]
[DOI]
(I p)
Boris Görke, Elodie Foulquier, Anne Galinier
YvcK of Bacillus subtilis is required for a normal cell shape and for growth on Krebs cycle intermediates and substrates of the pentose phosphate pathway.
Microbiology (Reading): 2005, 151(Pt 11);3777-3791
[PubMed:16272399]
[WorldCat.org]
[DOI]
(P p)
A Galinier, J Haiech, M C Kilhoffer, M Jaquinod, J Stülke, J Deutscher, I Martin-Verstraete
The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression.
Proc Natl Acad Sci U S A: 1997, 94(16);8439-44
[PubMed:9237995]
[WorldCat.org]
[DOI]
(P p)