TagF

• Description: CDP-glycerol:polyglycerol phosphate glycero-phosphotransferase
  
  

Gene name	tagF
Synonyms	rodC
Essential	yes PubMed
Product	CDP-glycerol:polyglycerol phosphate glycero-phosphotransferase
Function	biosynthesis of teichoic acid
Gene expression levels in SubtiExpress: tagF
Metabolic function and regulation of this protein in SubtiPathways: TagF
MW, pI	87 kDa, 9.4
Gene length, protein length	2238 bp, 746 aa
Immediate neighbours	tagG, tagE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, essential genes, membrane proteins

This gene is a member of the following regulons

PhoP regulon, WalR regulon

The gene

Basic information

• Locus tag: BSU35720

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU35720

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: CDP-glycerol + (glycerophosphate)(n) = CMP + (glycerophosphate)(n+1) (according to Swiss-Prot)

• Protein family: CDP-glycerol glycerophosphotransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • membrane associated PubMed

Database entries

• BsubCyc: BSU35720

• Structure:

• UniProt: P13485

• KEGG entry: [3]

• E.C. number: 2.7.8.12

Expression and regulation

• Operon: tagD-tagE-tagF PubMed

• Expression browser: tagF PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by phosphate (PhoP) PubMed
  • strongly repressed in response to glucose starvation in M9 medium PubMed

• Regulatory mechanism:
  • PhoP: transcription repression PubMed
  • WalR: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 168 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 693 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: