TagD

• Description: glycerol-3-phosphate cytidylyltransferase
  
  

Gene name	tagD
Synonyms
Essential	yes PubMed
Product	glycerol-3-phosphate cytidylyltransferase
Function	biosynthesis of teichoic acid
Gene expression levels in SubtiExpress: tagD
Metabolic function and regulation of this protein in SubtiPathways: TagD
MW, pI	15 kDa, 5.672
Gene length, protein length	387 bp, 129 aa
Immediate neighbours	tagE, tagA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, essential genes

This gene is a member of the following regulons

PhoP regulon, WalR regulon

The gene

Basic information

• Locus tag: BSU35740

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU35740

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol (according to Swiss-Prot)

• Protein family: cytidylyltransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • TagD-TagD PubMed

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU35740

• Structure: 1N1D (complex with CDP-glycerol) PubMed, 1COZ

• UniProt: P27623

• KEGG entry: [3]

• E.C. number: 2.7.7.39

Expression and regulation

• Operon: tagD-tagE-tagF PubMed

• Expression browser: tagD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by phosphate (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription repression PubMed
  • WalR: transcription activation PubMed

• Additional information:
  • The mRNA has a long 5' leader region. This may indicate RNA-based regulation PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 604 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 2215 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 751 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 656 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 811 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Alistair Howell, Sarah Dubrac, Kasper Krogh Andersen, David Noone, Juliette Fert, Tarek Msadek, Kevin Devine
Genes controlled by the essential YycG/YycF two-component system of Bacillus subtilis revealed through a novel hybrid regulator approach.
Mol Microbiol: 2003, 49(6);1639-55
[PubMed:12950927] [WorldCat.org] [DOI] (P p)

A P Bhavsar, T J Beveridge, E D Brown
Precise deletion of tagD and controlled depletion of its product, glycerol 3-phosphate cytidylyltransferase, leads to irregular morphology and lysis of Bacillus subtilis grown at physiological temperature.
J Bacteriol: 2001, 183(22);6688-93
[PubMed:11673441] [WorldCat.org] [DOI] (P p)

C H Weber, Y S Park, S Sanker, C Kent, M L Ludwig
A prototypical cytidylyltransferase: CTP:glycerol-3-phosphate cytidylyltransferase from bacillus subtilis.
Structure: 1999, 7(9);1113-24
[PubMed:10508782] [WorldCat.org] [DOI] (P p)

Y Qi, F M Hulett
Role of Pho-P in transcriptional regulation of genes involved in cell wall anionic polymer biosynthesis in Bacillus subtilis.
J Bacteriol: 1998, 180(15);4007-10
[PubMed:9683503] [WorldCat.org] [DOI] (P p)

W Liu, S Eder, F M Hulett
Analysis of Bacillus subtilis tagAB and tagDEF expression during phosphate starvation identifies a repressor role for PhoP-P.
J Bacteriol: 1998, 180(3);753-8
[PubMed:9457886] [WorldCat.org] [DOI] (P p)

C Mauël, A Bauduret, C Chervet, S Beggah, D Karamata
In Bacillus subtilis 168, teichoic acid of the cross-wall may be different from that of the cylinder: a hypothesis based on transcription analysis of tag genes.
Microbiology (Reading): 1995, 141 ( Pt 10);2379-89
[PubMed:7581998] [WorldCat.org] [DOI] (P p)

A L Honeyman, G C Stewart
The nucleotide sequence of the rodC operon of Bacillus subtilis.
Mol Microbiol: 1989, 3(9);1257-68
[PubMed:2507871] [WorldCat.org] [DOI] (P p)